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. 1990 Jun;100(2):261–268. doi: 10.1111/j.1476-5381.1990.tb15793.x

GABAA receptor-mediated increase in membrane chloride conductance in rat paratracheal neurones.

T G Allen 1, G Burnstock 1
PMCID: PMC1917423  PMID: 1696153

Abstract

1. The actions of gamma-aminobutyric acid (GABA) on the intramural neurones of 14-18 day old rats were studied in situ by use of intracellular current- and voltage-clamp techniques. The ionic conductance changes and the effects of various GABA-receptor agonists and antagonists on these neurones were also investigated. 2. Prolonged application of GABA either by ionophoresis (10 pC-10 nC) or superfusion (10-100 microM), evoked a biphasic membrane depolarization in over 90% of all paratracheal neurones studied. Typically, the response consisted of an initial rapid depolarization (18-45 ms) that subsequently faded over a period of 15-25 s to reveal a second smaller depolarization which was maintained for the duration of GABA application. Both components of the evoked response resulted in an increase in membrane conductance and an inward flow of current. 3. The amplitude of the transient inward current, recorded during the initial phase of the response, was linearly related to the membrane potential at which it was elicited and reversed symmetrically at a membrane potential of -32.7 mV. The underlying increase in conductance was largely independent of membrane potential. The equilibrium potential for the sustained inward current was -38.7 mV. Replacement of extracellular chloride with gluconate ions initially enhanced the GABA-evoked inward current. With successive applications of GABA in low chloride, the evoked current and conductance changes declined markedly. 4. Muscimol superfusion (1-10 microM) or ionophoresis (10 pC-10 nC) mimicked both the initial and late phases of the GABA-induced conductance change and inward current.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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