Abstract
1. Porcine neuromedin U-8 produced a concentration (0.3 nM-1 microM)-dependent contraction of the longitudinal muscle of the human isolated ileum, which was unaffected by either atropine (1 microM) or tetrodotoxin (1 microM). 2. By contrast, neuromedin U-8 had only a weak effect on the circular muscle of the human isolated ileum. 3. Neuromedin U-8 also produced a concentration-dependent contraction of mucosa-free muscle strips from the dome of the human isolated urinary bladder, its action being unaffected by either atropine or tetrodotoxin. 4. These findings indicate that neuromedin U-8 exerts a direct contractile effect on smooth muscle of the human intestinal and urinary tract.
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Selected References
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- Augood S. J., Keast J. R., Emson P. C. Distribution and characterisation of neuromedin U-like immunoreactivity in rat brain and intestine and in guinea pig intestine. Regul Pept. 1988 Apr;20(4):281–292. doi: 10.1016/0167-0115(88)90063-8. [DOI] [PubMed] [Google Scholar]
- Ballesta J., Carlei F., Bishop A. E., Steel J. H., Gibson S. J., Fahey M., Hennessey R., Domin J., Bloom S. R., Polak J. M. Occurrence and developmental pattern of neuromedin U-immunoreactive nerves in the gastrointestinal tract and brain of the rat. Neuroscience. 1988 Jun;25(3):797–816. doi: 10.1016/0306-4522(88)90037-1. [DOI] [PubMed] [Google Scholar]
- Brown D. R., Quito F. L. Neuromedin U octapeptide alters ion transport in porcine jejunum. Eur J Pharmacol. 1988 Oct 11;155(1-2):159–162. doi: 10.1016/0014-2999(88)90415-3. [DOI] [PubMed] [Google Scholar]
- Domin J., Ghatei M. A., Chohan P., Bloom S. R. Characterization of neuromedin U like immunoreactivity in rat, porcine, guinea-pig and human tissue extracts using a specific radioimmunoassay. Biochem Biophys Res Commun. 1986 Nov 14;140(3):1127–1134. doi: 10.1016/0006-291x(86)90752-7. [DOI] [PubMed] [Google Scholar]
- Domin J., Ghatei M. A., Chohan P., Bloom S. R. Neuromedin U--a study of its distribution in the rat. Peptides. 1987 Sep-Oct;8(5):779–784. doi: 10.1016/0196-9781(87)90058-1. [DOI] [PubMed] [Google Scholar]
- Maggi C. A., Giuliani S., Patacchini R., Santicioli P., Turini D., Barbanti G., Meli A. Potent contractile activity of endothelin on the human isolated urinary bladder. Br J Pharmacol. 1989 Apr;96(4):755–757. doi: 10.1111/j.1476-5381.1989.tb11879.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maggi C. A., Patacchini R., Santicioli P., Giuliani S., Turini D., Barbanti G., Beneforti P., Misuri D., Meli A. Human isolated small intestine: motor responses of the longitudinal muscle to field stimulation and exogenous neuropeptides. Naunyn Schmiedebergs Arch Pharmacol. 1989 Apr;339(4):415–423. doi: 10.1007/BF00736056. [DOI] [PubMed] [Google Scholar]
- Maggi C. A., Patacchini R., Santicioli P., Giuliani S., Turini D., Barbanti G., Beneforti P., Misuri D., Meli A. Specific motor effects of capsaicin on human jejunum. Eur J Pharmacol. 1988 May 10;149(3):393–395. doi: 10.1016/0014-2999(88)90676-0. [DOI] [PubMed] [Google Scholar]
- Maggi C. A., Santicioli P., Patacchini R., Cellerini M., Turini D., Barbanti G., Beneforti P., Rovero P., Meli A. Contractile response of the human isolated urinary bladder to neurokinins: involvement of NK-2 receptors. Eur J Pharmacol. 1988 Jan 19;145(3):335–340. doi: 10.1016/0014-2999(88)90438-4. [DOI] [PubMed] [Google Scholar]
- Minamino N., Kangawa K., Matsuo H. Neuromedin U-8 and U-25: novel uterus stimulating and hypertensive peptides identified in porcine spinal cord. Biochem Biophys Res Commun. 1985 Aug 15;130(3):1078–1085. doi: 10.1016/0006-291x(85)91726-7. [DOI] [PubMed] [Google Scholar]
- Sumi S., Inoue K., Kogire M., Doi R., Takaori K., Suzuki T., Yajima H., Tobe T. Effect of synthetic neuromedin U-8 and U-25, novel peptides identified in porcine spinal cord, on splanchnic circulation in dogs. Life Sci. 1987 Sep 28;41(13):1585–1590. doi: 10.1016/0024-3205(87)90725-9. [DOI] [PubMed] [Google Scholar]