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. 1997 Jul;71(7):5173–5177. doi: 10.1128/jvi.71.7.5173-5177.1997

Specific cytotoxic T lymphocytes are involved in in vivo clearance of infectious bronchitis virus.

S H Seo 1, E W Collisson 1
PMCID: PMC191752  PMID: 9188584

Abstract

Cytotoxic T-lymphocyte (CTL) responses to infectious bronchitis virus (IBV) were determined at regular intervals between 3 and 30 days postinfection (p.i.). The maximum response with 82% lysis of labeled target cells was detected at 10 days p.i. The specific CTL response did not begin to decline until the amount of virus, which peaked at day 8 p.i. in both the kidneys and lungs, started to decrease. Clinical respiratory signs of illness also correlated with amount of virus. CTL activity was shown to be major histocompatibility complex (MHC) class restricted because the lysis of MHC-mismatched targets was negligible, and lysis was mediated by CD8+ CD4- T cells, as the CTL response could be abolished with removal of CD8+ CD4- but not CD4+ CD8- lymphocytes. In contrast, immunoglobulin M (IgM) antibody was not detected until day 10 p.i., and levels peaked at day 12 p.i.; IgG antibody levels were minimal until day 15 p.i. but continued to increase exponentially until day 30 p.i., the last day examined. In summary, CTL responses correlated with initial decreases in infection and illness.

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Selected References

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  1. Allen G., Yeargan M., Costa L. R., Cross R. Major histocompatibility complex class I-restricted cytotoxic T-lymphocyte responses in horses infected with equine herpesvirus 1. J Virol. 1995 Jan;69(1):606–612. doi: 10.1128/jvi.69.1.606-612.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boots A. M., Kusters J. G., van Noort J. M., Zwaagstra K. A., Rijke E., van der Zeijst B. A., Hensen E. J. Localization of a T-cell epitope within the nucleocapsid protein of avian coronavirus. Immunology. 1991 Sep;74(1):8–13. [PMC free article] [PubMed] [Google Scholar]
  3. Boots A. M., Van Lierop M. J., Kusters J. G., Van Kooten P. J., Van der Zeijst B. A., Hensen E. J. MHC class II-restricted T-cell hybridomas recognizing the nucleocapsid protein of avian coronavirus IBV. Immunology. 1991 Jan;72(1):10–14. [PMC free article] [PubMed] [Google Scholar]
  4. Butcher G. D., Winterfield R. W., Shapiro D. P. An outbreak of nephropathogenic H13 infectious bronchitis in commercial broilers. Avian Dis. 1989 Oct-Dec;33(4):823–826. [PubMed] [Google Scholar]
  5. Chan M. M., Chen C. L., Ager L. L., Cooper M. D. Identification of the avian homologues of mammalian CD4 and CD8 antigens. J Immunol. 1988 Apr 1;140(7):2133–2138. [PubMed] [Google Scholar]
  6. Collisson E. W., Li J. Z., Sneed L. W., Peters M. L., Wang L. Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA. Vet Microbiol. 1990 Sep;24(3-4):261–271. doi: 10.1016/0378-1135(90)90176-V. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cook J. K., Davison T. F., Huggins M. B., McLaughlan P. Effect of in ovo bursectomy on the course of an infectious bronchitis virus infection in line C White Leghorn chickens. Arch Virol. 1991;118(3-4):225–234. doi: 10.1007/BF01314032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cumming R. B. The control of avian infectious bronchitis/nephrosis in Australia. Aust Vet J. 1969 Apr;45(4):200–203. doi: 10.1111/j.1751-0813.1969.tb01930.x. [DOI] [PubMed] [Google Scholar]
  9. Digby M. R., Lowenthal J. W. Cloning and expression of the chicken interferon-gamma gene. J Interferon Cytokine Res. 1995 Nov;15(11):939–945. doi: 10.1089/jir.1995.15.939. [DOI] [PubMed] [Google Scholar]
  10. Doherty P. C., Blanden R. V., Zinkernagel R. M. Specificity of virus-immune effector T cells for H-2K or H-2D compatible interactions: implications for H-antigen diversity. Transplant Rev. 1976;29:89–124. doi: 10.1111/j.1600-065x.1976.tb00198.x. [DOI] [PubMed] [Google Scholar]
  11. Glick B. Morphological changes and humoral immunity in cyclophosphamide-treated chicks. Transplantation. 1971 May;11(5):433–439. doi: 10.1097/00007890-197105000-00001. [DOI] [PubMed] [Google Scholar]
  12. Granfors K., Martin C., Lassila O., Suvitaival R., Toivanen A., Toivanen P. Immune capacity of the chicken bursectomized at 60 hr of incubation; production of the immunoglobulins and specific antibodies. Clin Immunol Immunopathol. 1982 May;23(2):459–469. doi: 10.1016/0090-1229(82)90130-1. [DOI] [PubMed] [Google Scholar]
  13. Herberman R. B., Reynolds C. W., Ortaldo J. R. Mechanism of cytotoxicity by natural killer (NK) cells. Annu Rev Immunol. 1986;4:651–680. doi: 10.1146/annurev.iy.04.040186.003251. [DOI] [PubMed] [Google Scholar]
  14. Kinde H., Daft B. M., Castro A. E., Bickford A. A., Gelb J., Jr, Reynolds B. Viral pathogenesis of a nephrotropic infectious bronchitis virus isolated from commercial pullets. Avian Dis. 1991 Apr-Jun;35(2):415–421. [PubMed] [Google Scholar]
  15. Kogut M. H., Lange C. Recombinant interferon-gamma inhibits cell invasion by Eimeria tenella. J Interferon Res. 1989 Feb;9(1):67–77. doi: 10.1089/jir.1989.9.67. [DOI] [PubMed] [Google Scholar]
  16. Lin Y. L., Askonas B. A. Biological properties of an influenza A virus-specific killer T cell clone. Inhibition of virus replication in vivo and induction of delayed-type hypersensitivity reactions. J Exp Med. 1981 Aug 1;154(2):225–234. doi: 10.1084/jem.154.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maccubbin D. L., Schierman L. W. MHC-restricted cytotoxic response of chicken T cells: expression, augmentation, and clonal characterization. J Immunol. 1986 Jan;136(1):12–16. [PubMed] [Google Scholar]
  18. Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
  19. McGuire T. C., Tumas D. B., Byrne K. M., Hines M. T., Leib S. R., Brassfield A. L., O'Rourke K. I., Perryman L. E. Major histocompatibility complex-restricted CD8+ cytotoxic T lymphocytes from horses with equine infectious anemia virus recognize Env and Gag/PR proteins. J Virol. 1994 Mar;68(3):1459–1467. doi: 10.1128/jvi.68.3.1459-1467.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McNeal M. M., Barone K. S., Rae M. N., Ward R. L. Effector functions of antibody and CD8+ cells in resolution of rotavirus infection and protection against reinfection in mice. Virology. 1995 Dec 20;214(2):387–397. doi: 10.1006/viro.1995.0048. [DOI] [PubMed] [Google Scholar]
  21. Offit P. A., Dudzik K. I. Rotavirus-specific cytotoxic T lymphocytes appear at the intestinal mucosal surface after rotavirus infection. J Virol. 1989 Aug;63(8):3507–3512. doi: 10.1128/jvi.63.8.3507-3512.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reusser P., Riddell S. R., Meyers J. D., Greenberg P. D. Cytotoxic T-lymphocyte response to cytomegalovirus after human allogeneic bone marrow transplantation: pattern of recovery and correlation with cytomegalovirus infection and disease. Blood. 1991 Sep 1;78(5):1373–1380. [PubMed] [Google Scholar]
  23. Sick C., Schultz U., Staeheli P. A family of genes coding for two serologically distinct chicken interferons. J Biol Chem. 1996 Mar 29;271(13):7635–7639. doi: 10.1074/jbc.271.13.7635. [DOI] [PubMed] [Google Scholar]
  24. Sneed L. W., Butcher G. D., Parr R., Wang L., Collisson E. W. Comparisons of the structural proteins of avian infectious bronchitis virus as determined by western blot analysis. Viral Immunol. 1989 Fall;2(3):221–227. doi: 10.1089/vim.1989.2.221. [DOI] [PubMed] [Google Scholar]
  25. Sussman M. A., Shubin R. A., Kyuwa S., Stohlman S. A. T-cell-mediated clearance of mouse hepatitis virus strain JHM from the central nervous system. J Virol. 1989 Jul;63(7):3051–3056. doi: 10.1128/jvi.63.7.3051-3056.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tashiro M., Fujii Y., Nakamura K., Homma M. Cell-mediated immunity induced in mice after vaccination with a protease activation mutant, TR-2, of Sendai virus. J Virol. 1988 Jul;62(7):2490–2497. doi: 10.1128/jvi.62.7.2490-2497.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thacker E. L., Fulton J. E., Hunt H. D. In vitro analysis of a primary, major histocompatibility complex (MHC)-restricted, cytotoxic T-lymphocyte response to avian leukosis virus (ALV), using target cells expressing MHC class I cDNA inserted into a recombinant ALV vector. J Virol. 1995 Oct;69(10):6439–6444. doi: 10.1128/jvi.69.10.6439-6444.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Townsend A., Bodmer H. Antigen recognition by class I-restricted T lymphocytes. Annu Rev Immunol. 1989;7:601–624. doi: 10.1146/annurev.iy.07.040189.003125. [DOI] [PubMed] [Google Scholar]
  29. Trinchieri G. Biology of natural killer cells. Adv Immunol. 1989;47:187–376. doi: 10.1016/S0065-2776(08)60664-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wang L., Junker D., Collisson E. W. Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology. 1993 Feb;192(2):710–716. doi: 10.1006/viro.1993.1093. [DOI] [PubMed] [Google Scholar]
  31. Weinstock D., Schat K. A., Calnek B. W. Cytotoxic T lymphocytes in reticuloendotheliosis virus-infected chickens. Eur J Immunol. 1989 Feb;19(2):267–272. doi: 10.1002/eji.1830190208. [DOI] [PubMed] [Google Scholar]
  32. Yamaguchi K., Kyuwa S., Nakanaga K., Hayami M. Establishment of cytotoxic T-cell clones specific for cells infected with mouse hepatitis virus. J Virol. 1988 Jul;62(7):2505–2507. doi: 10.1128/jvi.62.7.2505-2507.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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