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. 1990 Apr;99(4):709–712. doi: 10.1111/j.1476-5381.1990.tb12993.x

Subclassification of beta-adrenoceptors responsible for steroidogenesis in primary cultures of bovine adrenocortical zona fasciculata/reticularis cells.

E R Lightly 1, S W Walker 1, I M Bird 1, B C Williams 1
PMCID: PMC1917547  PMID: 1972892

Abstract

1. Forty eight hour primary cultures of purified bovine adrenocortical zona fasciculata/reticularis cells secreted hydrocortisone in response to stimulation with beta-adrenoceptor agonists. The observed order of potency was isoprenaline greater than noradrenaline greater than dobutamine greater than salbutamol greater than BRL37344. 2. Salbutamol acted as a partial agonist on these cells hence suggesting the presence of a beta 1-adrenoceptor. 3. Schild analysis of the hydrocortisone response to isoprenaline showed that the selective beta 1-antagonist practolol and the selective beta 2-antagonist ICI118,551 gave pA2 values of 6.85 and 7.17, respectively. These values were in close agreement with corresponding pA2 values previously obtained for the beta 1-adrenoceptor. 4. We conclude that beta 1-adrenoceptors are responsible for mediating catecholamine-stimulated hydrocortisone secretion from primary cultures of bovine zona fasciculata/reticularis cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arch J. R., Ainsworth A. T., Cawthorne M. A., Piercy V., Sennitt M. V., Thody V. E., Wilson C., Wilson S. Atypical beta-adrenoceptor on brown adipocytes as target for anti-obesity drugs. Nature. 1984 May 10;309(5964):163–165. doi: 10.1038/309163a0. [DOI] [PubMed] [Google Scholar]
  2. Bilski A. J., Halliday S. E., Fitzgerald J. D., Wale J. L. The pharmacology of a beta 2-selective adrenoceptor antagonist (ICI 118,551). J Cardiovasc Pharmacol. 1983 May-Jun;5(3):430–437. doi: 10.1097/00005344-198305000-00013. [DOI] [PubMed] [Google Scholar]
  3. Bond R. A., Clarke D. E. Agonist and antagonist characterization of a putative adrenoceptor with distinct pharmacological properties from the alpha- and beta-subtypes. Br J Pharmacol. 1988 Nov;95(3):723–734. doi: 10.1111/j.1476-5381.1988.tb11698.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Léan A., Racz K., McNicoll N., Desrosiers M. L. Direct beta-adrenergic stimulation of aldosterone secretion in cultured bovine adrenal subcapsular cells. Endocrinology. 1984 Aug;115(2):485–492. doi: 10.1210/endo-115-2-485. [DOI] [PubMed] [Google Scholar]
  5. Dunlop D., Shanks R. G. Selective blockade of adrenoceptive beta receptors in the heart. Br J Pharmacol Chemother. 1968 Jan;32(1):201–218. doi: 10.1111/j.1476-5381.1968.tb00444.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farmer J. B., Kennedy I., Levy G. P., Marshall R. J. A comparison of the beta-adrenoreceptor stimulant properties of isoprenaline, with those of orciprenaline, salbutamol, soterenol and trimetoquinol on isolated atria and trachea of the guinea pig. J Pharm Pharmacol. 1970 Jan;22(1):61–63. doi: 10.1111/j.2042-7158.1970.tb08388.x. [DOI] [PubMed] [Google Scholar]
  7. Gill G. N., Ill C. R., Simonian M. H. Angiotensin stimulation of bovine adrenocortical cell growth. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5569–5573. doi: 10.1073/pnas.74.12.5569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaumann A. J. Is there a third heart beta-adrenoceptor? Trends Pharmacol Sci. 1989 Aug;10(8):316–320. doi: 10.1016/0165-6147(89)90065-5. [DOI] [PubMed] [Google Scholar]
  9. Kawamura M., Nakamichi N., Imagawa N., Tanaka Y., Tomita C., Matsuba M. Effect of adrenaline on steroidogenesis in primary cultured bovine adrenocortical cells. Jpn J Pharmacol. 1984 Sep;36(1):35–41. doi: 10.1254/jjp.36.35. [DOI] [PubMed] [Google Scholar]
  10. Kenakin T. P., Black J. W. The pharmacological classification of practolol and chloropractolol. Mol Pharmacol. 1978 Jul;14(4):607–623. [PubMed] [Google Scholar]
  11. Kenakin T. P. The Schild regression in the process of receptor classification. Can J Physiol Pharmacol. 1982 Mar;60(3):249–265. doi: 10.1139/y82-036. [DOI] [PubMed] [Google Scholar]
  12. Kleitman N., Holzwarth M. A. Catecholaminergic innervation of the rat adrenal cortex. Cell Tissue Res. 1985;241(1):139–147. doi: 10.1007/BF00214635. [DOI] [PubMed] [Google Scholar]
  13. Lands A. M., Arnold A., McAuliff J. P., Luduena F. P., Brown T. G., Jr Differentiation of receptor systems activated by sympathomimetic amines. Nature. 1967 May 6;214(5088):597–598. doi: 10.1038/214597a0. [DOI] [PubMed] [Google Scholar]
  14. McDougall J. G., Williams B. C., Hyatt P. J., Bell J. B., Tait J. F., Tait S. A. Purification of dispersed rat adrenal cells by column filtration. Proc R Soc Lond B Biol Sci. 1979 Nov 30;206(1162):15–32. doi: 10.1098/rspb.1979.0089. [DOI] [PubMed] [Google Scholar]
  15. Migally N. The innervation of the mouse adrenal cortex. Anat Rec. 1979 May;194(1):105–111. doi: 10.1002/ar.1091940107. [DOI] [PubMed] [Google Scholar]
  16. Mikhail Y., Amin F. Intrinsic innervation of the human adrenal gland. Acta Anat (Basel) 1969;72(1):25–32. doi: 10.1159/000143232. [DOI] [PubMed] [Google Scholar]
  17. Shima S., Komoriyama K., Hirai M., Kouyama H. Studies on cyclic nucleotides in the adrenal gland. XI. Adrenergic regulation of adenylate cyclase activity in the adrenal cortex. Endocrinology. 1984 Feb;114(2):325–329. doi: 10.1210/endo-114-2-325. [DOI] [PubMed] [Google Scholar]
  18. Ungar A. The cardiovascular actions of the beta-adrenoceptor antagonists. Eur J Clin Invest. 1979 Jun;9(3):175–177. doi: 10.1111/j.1365-2362.1979.tb00918.x. [DOI] [PubMed] [Google Scholar]
  19. Walker S. W., Lightly E. R., Milner S. W., Williams B. C. Catecholamine stimulation of cortisol secretion by 3-day primary cultures of purified zona fasciculata/reticularis cells isolated from bovine adrenal cortex. Mol Cell Endocrinol. 1988 May;57(1-2):139–147. doi: 10.1016/0303-7207(88)90043-3. [DOI] [PubMed] [Google Scholar]
  20. Williams B. C., Lightly E. R., Ross A. R., Bird I. M., Walker S. W. Characterization of the steroidogenic responsiveness and ultrastructure of purified zona fasciculata/reticularis cells from bovine adrenal cortex before and after primary culture. J Endocrinol. 1989 May;121(2):317–324. doi: 10.1677/joe.0.1210317. [DOI] [PubMed] [Google Scholar]

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