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. 1990 Dec;101(4):889–895. doi: 10.1111/j.1476-5381.1990.tb14176.x

Prostanoid stimulation of anion secretion in guinea-pig gastric and ileal mucosa is mediated by different receptors.

K T Bunce 1, C F Spraggs 1
PMCID: PMC1917834  PMID: 2085712

Abstract

1. The receptors that mediate stimulation of anion secretion by prostanoids in isolated preparations of guinea-pig gastric and ileal mucosa have been compared by use of selective prostanoid agonists and antagonists. 2. In gastric mucosa, the relative potency of agonists suggested that the control of anion secretion in this tissue was complex and may be mediated by EP2, EP3, and TP receptors. A role for TP receptors was confirmed with the TP-selective antagonist AH23848 which inhibited short circuit current responses to the TP receptor agonist U-46619 with a pA2 value of 8.44 but was without effect on responses to prostaglandin E2 (PGE2) or the EP selective agonist, sulprostone. 3. In ileal mucosa, the relative potency of agonists differed from that observed in gastric mucosa and was consistent with the view that anion secretion in this region of intestine was controlled by DP and EP2 receptors. 4. These studies suggest that anion secretion in gastric and ileal mucosa is controlled by different prostanoid receptor subtypes and so provide important information for the design of prostanoids which may protect gastric mucosa and that are free from side effects such as diarrhoea.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen A., Garner A. Mucus and bicarbonate secretion in the stomach and their possible role in mucosal protection. Gut. 1980 Mar;21(3):249–262. doi: 10.1136/gut.21.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baird A. W., Coombs R. R., McLaughlan P., Cuthbert A. W. Immediate hypersensitivity reactions to cow milk proteins in isolated epithelium from ileum of milk-drinking guinea-pigs: comparisons with colonic epithelia. Int Arch Allergy Appl Immunol. 1984;75(3):255–263. doi: 10.1159/000233625. [DOI] [PubMed] [Google Scholar]
  3. Brand D. L., Roufail W. M., Thomson A. B., Tapper E. J. Misoprostol, a synthetic PGE1 analog, in the treatment of duodenal ulcers. A multicenter double-blind study. Dig Dis Sci. 1985 Nov;30(11 Suppl):147S–158S. doi: 10.1007/BF01309402. [DOI] [PubMed] [Google Scholar]
  4. Brittain R. T., Boutal L., Carter M. C., Coleman R. A., Collington E. W., Geisow H. P., Hallett P., Hornby E. J., Humphrey P. P., Jack D. AH23848: a thromboxane receptor-blocking drug that can clarify the pathophysiologic role of thromboxane A2. Circulation. 1985 Dec;72(6):1208–1218. doi: 10.1161/01.cir.72.6.1208. [DOI] [PubMed] [Google Scholar]
  5. Bunce K. T., Clayton N. M. The effects of the stable thromboxane A2-mimetic, U46619, on gastric mucosal damage and gastric non-parietal secretion in the rat. Br J Pharmacol. 1987 May;91(1):23–29. doi: 10.1111/j.1476-5381.1987.tb08979.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bunce K. T., Spraggs C. F. Stimulation of electrogenic chloride secretion by prostaglandin E2 in guinea-pig isolated gastric mucosa. J Physiol. 1988 Jun;400:381–394. doi: 10.1113/jphysiol.1988.sp017126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coleman R. A., Kennedy I., Sheldrick R. L. New evidence with selective agonists and antagonists for the subclassification of PGE2-sensitive (EP) receptors. Adv Prostaglandin Thromboxane Leukot Res. 1987;17A:467–470. [PubMed] [Google Scholar]
  8. Gana T. J., MacPherson B. R., Ng D., Koo J. Ionic fluxes induced by topical misoprostol in canine gastric mucosa. Can J Physiol Pharmacol. 1989 Apr;67(4):353–358. doi: 10.1139/y89-056. [DOI] [PubMed] [Google Scholar]
  9. Jones R. L., Peesapati V., Wilson N. H. Antagonism of the thromboxane-sensitive contractile systems of the rabbit aorta, dog saphenous vein and guinea-pig trachea. Br J Pharmacol. 1982 Jul;76(3):423–438. doi: 10.1111/j.1476-5381.1982.tb09236.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kennedy I., Coleman R. A., Humphrey P. P., Levy G. P., Lumley P. Studies on the characterisation of prostanoid receptors: a proposed classification. Prostaglandins. 1982 Nov;24(5):667–689. doi: 10.1016/0090-6980(82)90036-3. [DOI] [PubMed] [Google Scholar]
  11. Lauritsen K., Laursen L. S., Havelund T., Bytzer P., Svendsen L. B., Rask-Madsen J. Enprostil and ranitidine in duodenal ulcer healing: double blind comparative trial. Br Med J (Clin Res Ed) 1986 Mar 29;292(6524):864–866. doi: 10.1136/bmj.292.6524.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller O. V., Gorman R. R. Evidence for distinct prostaglandin I2 and D2 receptors in human platelets. J Pharmacol Exp Ther. 1979 Jul;210(1):134–140. [PubMed] [Google Scholar]
  13. Miller T. A., Henagan J. M., Watkins L. A., Loy T. M. Prostaglandin-induced bicarbonate secretion in the canine stomach: characteristics and evidence for a cholinergic mechanism. J Surg Res. 1983 Aug;35(2):105–112. doi: 10.1016/0022-4804(83)90132-4. [DOI] [PubMed] [Google Scholar]
  14. Musch M. W., Field M., Miller R. J., Stoff J. S. Homologous desensitization to prostaglandins in rabbit ileum. Am J Physiol. 1987 Jan;252(1 Pt 1):G120–G127. doi: 10.1152/ajpgi.1987.252.1.G120. [DOI] [PubMed] [Google Scholar]
  15. Pihan G., Szabo S. Protection of gastric mucosa against hypertonic sodium chloride by 16,16-dimethyl prostaglandin E2 or sodium thiosulfate in the rat: evidence for decreased mucosal penetration of damaging agent. Dig Dis Sci. 1989 Dec;34(12):1865–1872. doi: 10.1007/BF01536704. [DOI] [PubMed] [Google Scholar]
  16. Racusen L. C., Binder H. J. Effect of prostaglandin on ion transport across isolated colonic mucosa. Dig Dis Sci. 1980 Dec;25(12):900–904. doi: 10.1007/BF01308038. [DOI] [PubMed] [Google Scholar]
  17. Robert A., Nezamis J. E., Lancaster C., Hanchar A. J. Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology. 1979 Sep;77(3):433–443. [PubMed] [Google Scholar]
  18. Roszkowski A. P., Garay G. L., Baker S., Schuler M., Carter H. Gastric antisecretory and antiulcer properties of enprostil, (+/-)-11 alpha, 15 alpha-dihydroxy-16-phenoxy-17,18,19,20-tetranor-9-oxoprosta- 4,5,13(t)-trienoic acid methyl ester. J Pharmacol Exp Ther. 1986 Nov;239(2):382–389. [PubMed] [Google Scholar]
  19. Sernka T. J., Rood R. P., Mah M. Y., Tseng C. H. Antiabsorptive effects of 16,16 dimethyl prostaglandin E2 in isolated rat colon. Prostaglandins. 1982 Mar;23(3):411–426. doi: 10.1016/0090-6980(82)90086-7. [DOI] [PubMed] [Google Scholar]

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