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. 1991 Feb;102(2):351–354. doi: 10.1111/j.1476-5381.1991.tb12177.x

Yohimbine increases submaxillary kallikrein release into the saliva in dogs: evidence for alpha 2-adrenoceptor-mediated inhibition of cholinergic pathways.

J P Girolami 1, J L Bascands 1, C Pécher 1, M Berlan 1, J L Montastruc 1, P Montastruc 1
PMCID: PMC1918049  PMID: 1849766

Abstract

1. The effects of the alpha 2-adrenoceptor antagonist, yohimbine (0.5 mg kg-1, i.v.) on basal, sympathetic and parasympathetic stimulation-induced submaxillary kallikrein release were investigated in the anaesthetized dog. Kallikrein was measured by its kininogenase activity before and after trypsin activation which also allowed a study of the proportion of active to total enzyme. 2. Yohimbine induced a rapid, three fold increase in basal kallikrein release correlated with an increase in salivary flow rate which lasted for 60 min following injection. 3. Sectioning the chorda tympani did not affect basal kallikrein release but abolished yohimbine-induced rise in salivary kallikrein secretion. 4. Parasympathetic stimulation alone induced a 3 to 4 fold increase in basal kallikrein release correlated with an increase in salivary flow rate. Yohimbine induced a significant additional increase in parasympathetic-stimulated kallikrein release. 5. When the cervical sympathetic nerve was sectioned the basal kallikrein release decreased by 30 to 40%. 6. Sympathetic stimulation alone also induced a 3 to 4 fold increase in basal kallikrein. This was not correlated with the salivary flow and unaffected by yohimbine. 7. The results indicate that yohimbine increases submaxillary kallikrein release into the saliva by inhibition of presynaptic alpha 2-adrenoceptors located on the chorda tympani nerve endings.

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Selected References

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  1. Alhenc-Gelas F., Marchetti J., Allegrini J., Corvol P., Menard J. Measurement of urinary kallikrein activity. Species differences in kinin production. Biochim Biophys Acta. 1981 Nov 5;677(3-4):477–488. doi: 10.1016/0304-4165(81)90262-2. [DOI] [PubMed] [Google Scholar]
  2. Berg T., Carretero O. A., Scicli A. G., Tilley B., Stewart J. M. Role of kinin in regulation of rat submandibular gland blood flow. Hypertension. 1989 Jul;14(1):73–80. doi: 10.1161/01.hyp.14.1.73. [DOI] [PubMed] [Google Scholar]
  3. Bylund D. B., Martinez J. R. Postsynaptic localization of alpha 2-adrenergic receptors in rat submandibular gland. J Neurosci. 1981 Sep;1(9):1003–1007. doi: 10.1523/JNEUROSCI.01-09-01003.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bylund D. B., Martinez J. R. alpha 2-Adrenergic receptors appear in rat salivary glands after reserpine treatment. Nature. 1980 May 22;285(5762):229–230. doi: 10.1038/285229a0. [DOI] [PubMed] [Google Scholar]
  5. Chatelut E., Rispail Y., Berlan M., Montastruc J. L. Yohimbine increases human salivary secretion. Br J Clin Pharmacol. 1989 Sep;28(3):366–368. doi: 10.1111/j.1365-2125.1989.tb05440.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fuller P. J., Funder J. W. The cellular physiology of glandular kallikrein. Kidney Int. 1986 May;29(5):953–964. doi: 10.1038/ki.1986.93. [DOI] [PubMed] [Google Scholar]
  7. Garrett J. R., Smith R. E., Kyriacou K., Kidd A., Liao J. Factors affecting the secretion of submandibular salivary kallikrein in cats. Q J Exp Physiol. 1987 Jul;72(3):357–368. doi: 10.1113/expphysiol.1987.sp003081. [DOI] [PubMed] [Google Scholar]
  8. Gautvik K., Kriz M., Lund-Larsen K., Waaler B. A. Sympathetic vasodilatation, kallikrein release and adrenergic receptors in the cat submandibular salivary gland. Acta Physiol Scand. 1974 Feb;90(2):438–444. doi: 10.1111/j.1748-1716.1974.tb05606.x. [DOI] [PubMed] [Google Scholar]
  9. Green G. J., Wilson H., Yates M. S. The effect of clonidine on centrally and peripherally evoked submaxillary salivation. Eur J Pharmacol. 1979 Jan 15;53(3):297–300. doi: 10.1016/0014-2999(79)90136-5. [DOI] [PubMed] [Google Scholar]
  10. Montastruc P., Berlan M., Montastruc J. L. Effects of yohimbine on submaxillary salivation in dogs. Br J Pharmacol. 1989 Sep;98(1):101–104. doi: 10.1111/j.1476-5381.1989.tb16868.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moore N. D., Chadelaud M., Huchet A. M., Saligaut C., Daoust M., Lhuintre J. P., Chrétien P., Boismare F. Urethane interferes with the alpha 2-agonist guanfacine. J Cardiovasc Pharmacol. 1984 Sep-Oct;6(5):973–974. doi: 10.1097/00005344-198409000-00036. [DOI] [PubMed] [Google Scholar]
  12. Orstavik T. B., Carretero O. A., Johansen L., Scicli A. G. Role of kallikrein in the hypertensive effect of captopril after sympathetic stimulation of the rat submandibular gland. Circ Res. 1982 Sep;51(3):385–390. doi: 10.1161/01.res.51.3.385. [DOI] [PubMed] [Google Scholar]
  13. Orstavik T. B., Carretero O. A., Scicli A. G. Kallikrein-kinin system in regulation of submandibular gland blood flow. Am J Physiol. 1982 Jun;242(6):H1010–H1014. doi: 10.1152/ajpheart.1982.242.6.H1010. [DOI] [PubMed] [Google Scholar]
  14. Orstavik T. B., Gautvik K. M. Regulation of salivary kallikrein secretion in submandibular gland. Acta Physiol Scand. 1977 May;100(1):33–44. doi: 10.1111/j.1748-1716.1977.tb05919.x. [DOI] [PubMed] [Google Scholar]
  15. Orstavik T. B. The distribution and secretion of kallikrein in some exocrine organs of the rat. Acta Physiol Scand. 1978 Dec;104(4):431–442. doi: 10.1111/j.1748-1716.1978.tb06298.x. [DOI] [PubMed] [Google Scholar]

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