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. 1994 Apr;54(4):631–642.

The Apolipoprotein E/CI/CII Gene Cluster and Late-Onset Alzheimer Disease

Chang-En Yu, Haydeh Payami, Jane M Olson, Michael Boehnke, Ellen M Wijsman, Harry T Orr, Walter A Kukull, Katrina A B Goddard, Ellen Nemens, June A White, M Elisa Alonso, Todd D Taylor, Melvyn J Ball, Jeffrey Kaye, John Morris, Helena Chui, Adele D Sadovnick, George M Martin, Eric B Larson, Leonard L Heston, Thomas D Bird, Gerard D Schellenberg
PMCID: PMC1918105  PMID: 8128960

Abstract

The chromosome 19 apolipoprotein E/CI/CII gene cluster was examined for evidence of linkage to a familial Alzheimer disease (FAD) locus. The family groups studied were Volga German (VG), early-onset non-VG (ENVG; mean age at onset <60 years), and late-onset families. A genetic association was observed between apolipoprotein E (ApoE) allele ε4 and FAD in late-onset families; the ε4 allele frequency was .51 in affected subjects, .37 in at-risk subjects, .11 in spouses, and .19 in unrelated controls. The differences between the ε4 frequencies in affected subjects versus controls and in at-risk subjects versus controls were highly significant (standard normal deviate [ZSND]) = 7.37, P < 10−9; and ZSND = 4.07, P < .00005, respectively). No association between the ε4 allele and FAD was observed in the ENVG or VG groups. A statistically significant allelic association between ε4 and AD was also observed in a group of unrelated subjects; the ε4 frequency was .26 in affected subjects, versus .19 in controls (ZSND = 2.20, P < .03). Evidence of linkage of ApoE and ApoCII to FAD was examined by maximum-likelihood methods, using three models and assuming autosomal dominant inheritance: (1) age-dependent penetrance, (2) extremely low (1%) penetrance, and (3) age-dependent penetrance corrected for sporadic Alzheimer disease (AD). For ApoCII in late-onset families, results for close linkage were negative, and only small positive lod-score-statistic (Z) values were obtained (model 1, maximum Z [Zmax] = 0.61, recombination fraction [θ] = .30; model 2, Zmax = 0.47, θ = .20). For ApoE in late-onset kindreds, positive Z values were obtained when either allele frequencies from controls (model 1, Zmax = 2.02, θ = .15; model 2, Zmax = 3.42, θ = .05) or allele frequencies from the families (model 1, Zmax = 1.43, θ = .15; model 2, Zmax = 1.70, θ = .05) were used. When linkage disequilibrium was incorporated into the analysis, the Z values increased (model 1, Zmax = 3.17, θ = .23; model 3, Zmax = 1.85, θ = .20). For the ENVG group, results for ApoE and ApoCII were uniformly negative. Affected-pedigree-member analysis gave significant results for the late-onset kindreds, for ApoE (ZSND = 3.003, P = .003) and ApoCII (ZSND = 2.319, P = .016), when control allele frequencies were used but not when allele frequencies were derived from the families.

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Selected References

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