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. 1994 Mar;54(3):526–534.

A Radiation Hybrid Map of the BRCA1 Region

P O'Connell, H Albertsen, N Matsunami, T Taylor, J E Hundley, T L Johnson-Pais, B Reus, E Lawrence, L Ballard, R White, R J Leach
PMCID: PMC1918134  PMID: 8116622

Abstract

A locus on chromosome 17q, designated “BRCA1,” has been identified as a predisposition gene for breast cancer. A panel of chromosome 17–specific radiation-reduced somatic cell hybrid clones has been assembled for high-resolution mapping of chromosome 17. A series of 35 markers, known to span the BRCA1 locus, were tested against this hybrid panel by PCR assays. Statistical analysis of these data yields a BRCA1 radiation hybrid map at a density sufficient to initiate YAC cloning and pulsed-field gel electrophoretic mapping of the candidate region. In addition, many of the markers reveal genetic polymorphisms and may be tested in breast cancer families and in loss-of-heterozygosity studies of sporadic breast cancers to better define the BRCA1 gene candidate region.

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Selected References

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