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. 1997 Jul;71(7):5692–5695. doi: 10.1128/jvi.71.7.5692-5695.1997

Human cytomegalovirus induces interleukin-8 production by a human monocytic cell line, THP-1, through acting concurrently on AP-1- and NF-kappaB-binding sites of the interleukin-8 gene.

T Murayama 1, Y Ohara 1, M Obuchi 1, K S Khabar 1, H Higashi 1, N Mukaida 1, K Matsushima 1
PMCID: PMC191819  PMID: 9188651

Abstract

Cytomegalovirus (CMV) infection induced interleukin-8 (IL-8) gene transcription in a human monocytic cell line, THP-1 cells, leading to IL-8 secretion. The functional analysis of the IL-8 gene revealed that both AP-1- and NF-kappaB factor-binding elements were involved in conferring the responsiveness to CMV. Moreover, electrophoretic mobility shift assays demonstrated that CMV induced the formation of NF-kappaB and AP-1 complexes. These results suggest that CMV activates these transcriptional factors, resulting in IL-8 gene expression.

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Selected References

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