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. 1992 Aug;36(8):1619–1625. doi: 10.1128/aac.36.8.1619

Innovative endpoint determination system for antifungal susceptibility testing of yeasts.

R Tellier 1, M Krajden 1, G A Grigoriew 1, I Campbell 1
PMCID: PMC192019  PMID: 1416843

Abstract

Fungal infections and antifungal resistance are increasingly recognized. Antifungal susceptibility testing remains unstandardized, and a particularly important problem is endpoint determination. In this paper we propose the yeast metabolic reduction of the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetraz olium hydroxide (XTT) as a colorimetric endpoint which is quantitative and objective. Amphotericin B, fluorocytosine, and fluconazole dose-response curves were obtained, and a metabolic MIC could be determined by using precise criteria.

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Selected References

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  1. Alley M. C., Scudiero D. A., Monks A., Hursey M. L., Czerwinski M. J., Fine D. L., Abbott B. J., Mayo J. G., Shoemaker R. H., Boyd M. R. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988 Feb 1;48(3):589–601. [PubMed] [Google Scholar]
  2. Altman F. P. Tetrazolium salts and formazans. Prog Histochem Cytochem. 1976;9(3):1–56. doi: 10.1016/s0079-6336(76)80015-0. [DOI] [PubMed] [Google Scholar]
  3. Comley J. C., Turner C. H. Potential of a soluble tetrazolium/formazan assay for the evaluation of filarial viability. Int J Parasitol. 1990 Apr;20(2):251–255. doi: 10.1016/0020-7519(90)90107-x. [DOI] [PubMed] [Google Scholar]
  4. Espinel-Ingroff A., Kerkering T. M., Goldson P. R., Shadomy S. Comparison study of broth macrodilution and microdilution antifungal susceptibility tests. J Clin Microbiol. 1991 Jun;29(6):1089–1094. doi: 10.1128/jcm.29.6.1089-1094.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ford C. H., Richardson V. J., Tsaltas G. Comparison of tetrazolium colorimetric and [3H]-uridine assays for in vitro chemosensitivity testing. Cancer Chemother Pharmacol. 1989;24(5):295–301. doi: 10.1007/BF00304761. [DOI] [PubMed] [Google Scholar]
  6. Galgiani J. N. Antifungal susceptibility tests. Antimicrob Agents Chemother. 1987 Dec;31(12):1867–1870. doi: 10.1128/aac.31.12.1867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gordon M. A., Lapa E. W., Passero P. G. Improved method for azole antifungal susceptibility testing. J Clin Microbiol. 1988 Sep;26(9):1874–1877. doi: 10.1128/jcm.26.9.1874-1877.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hadfield T. L., Smith M. B., Winn R. E., Rinaldi M. G., Guerra C. Mycoses caused by Candida lusitaniae. Rev Infect Dis. 1987 Sep-Oct;9(5):1006–1012. doi: 10.1093/clinids/9.5.1006. [DOI] [PubMed] [Google Scholar]
  9. Hughes C. E., Bennett R. L., Beggs W. H. Broth dilution testing of Candida albicans susceptibility to ketoconazole. Antimicrob Agents Chemother. 1987 Apr;31(4):643–646. doi: 10.1128/aac.31.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
  11. Pfaller M. A., Burmeister L., Bartlett M. S., Rinaldi M. G. Multicenter evaluation of four methods of yeast inoculum preparation. J Clin Microbiol. 1988 Aug;26(8):1437–1441. doi: 10.1128/jcm.26.8.1437-1441.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pfaller M. A., Rinaldi M. G., Galgiani J. N., Bartlett M. S., Body B. A., Espinel-Ingroff A., Fromtling R. A., Hall G. S., Hughes C. E., Odds F. C. Collaborative investigation of variables in susceptibility testing of yeasts. Antimicrob Agents Chemother. 1990 Sep;34(9):1648–1654. doi: 10.1128/aac.34.9.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scudiero D. A., Shoemaker R. H., Paull K. D., Monks A., Tierney S., Nofziger T. H., Currens M. J., Seniff D., Boyd M. R. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 1988 Sep 1;48(17):4827–4833. [PubMed] [Google Scholar]
  14. Shepherd M. G., Poulter R. T., Sullivan P. A. Candida albicans: biology, genetics, and pathogenicity. Annu Rev Microbiol. 1985;39:579–614. doi: 10.1146/annurev.mi.39.100185.003051. [DOI] [PubMed] [Google Scholar]
  15. Twentyman P. R., Fox N. E., Rees J. K. Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay. Br J Haematol. 1989 Jan;71(1):19–24. doi: 10.1111/j.1365-2141.1989.tb06268.x. [DOI] [PubMed] [Google Scholar]
  16. Weislow O. S., Kiser R., Fine D. L., Bader J., Shoemaker R. H., Boyd M. R. New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity. J Natl Cancer Inst. 1989 Apr 19;81(8):577–586. doi: 10.1093/jnci/81.8.577. [DOI] [PubMed] [Google Scholar]

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