Abstract
Methylobacterium species represent a relatively new genus which is being increasingly isolated from cases of opportunistic infections. This study reports on 3 reference strains and 15 clinical isolates of Methylobacterium species. Susceptibility tests were performed by the agar dilution and commercial broth microdilution methods at both 30 and 35 degrees C. Readings were made at 24, 48, and 72 h. Incubation conditions of 48 h and 30 degrees C were found to be optimum. Both the agar dilution and broth microdilution methods gave equivalent results. Drugs tested and their MICs for 90% of isolates (in micrograms per milliliter) were as follows: amikacin, less than or equal to 1; gentamicin, 1; ciprofloxacin, 1; trimethoprim-sulfamethoxazole, 2/38; ceftriaxone, 16; and ceftizoxime, 16. The majority of our isolates were resistant to six other beta-lactam drugs tested. Nine of the 15 Methylobacterium isolates were beta-lactamase positive.
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Selected References
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- Gilardi G. L., Faur Y. C. Pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria. J Clin Microbiol. 1984 Oct;20(4):626–629. doi: 10.1128/jcm.20.4.626-629.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilchrist M. J., Kraft J. A., Hammond J. G., Connelly B. L., Myers M. G. Detection of Pseudomonas mesophilica as a source of nosocomial infections in a bone marrow transplant unit. J Clin Microbiol. 1986 Jun;23(6):1052–1055. doi: 10.1128/jcm.23.6.1052-1055.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutherford P. C., Narkowicz J. E., Wood C. J., Peel M. M. Peritonitis caused by Pseudomonas mesophilica in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol. 1988 Nov;26(11):2441–2443. doi: 10.1128/jcm.26.11.2441-2443.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith S. M., Eng R. H., Forrester C. Pseudomonas mesophilica infections in humans. J Clin Microbiol. 1985 Mar;21(3):314–317. doi: 10.1128/jcm.21.3.314-317.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallace P. L., Hollis D. G., Weaver R. E., Moss C. W. Biochemical and chemical characterization of pink-pigmented oxidative bacteria. J Clin Microbiol. 1990 Apr;28(4):689–693. doi: 10.1128/jcm.28.4.689-693.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welch D. F. Applications of cellular fatty acid analysis. Clin Microbiol Rev. 1991 Oct;4(4):422–438. doi: 10.1128/cmr.4.4.422. [DOI] [PMC free article] [PubMed] [Google Scholar]