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Journal of Virology logoLink to Journal of Virology
. 1997 Oct;71(10):7657–7662. doi: 10.1128/jvi.71.10.7657-7662.1997

Foot-and-mouth disease virus and poliovirus particles contain proteins of the replication complex.

J F Newman 1, F Brown 1
PMCID: PMC192115  PMID: 9311848

Abstract

Nonstructural proteins 2C, 3CD, 3C, and 3D, and the cellular protein actin, are present in highly purified preparations of foot-and-mouth disease virus (FMDV) and poliovirus. They remain bound in variable amounts to the RNAs when the RNAs are extracted from the viruses with phenol or phenol-sodium dodecyl sulfate (SDS) and, for FMDV, when the RNA is released from the particles by a lowering of the pH below 7. RNA prepared by these methods is rapidly degraded at 37 degrees C, particularly in the presence of NH4+ ions, but hydrolysis can be prevented by antibody against Escherichia coli-expressed 3D, indicating that it is the RNA polymerase that has nuclease activity. In contrast, virion RNA from which the nonstructural proteins and actin have been removed by extraction with guanidine thiocyanate-phenol-chloroform or proteinase K-phenol is stable at 37 degrees C, although its specific infectivity is lower than that of the RNA extracted with phenol or phenol-SDS. The possible implications of the close association of replication complex proteins with the RNA in virus particles are discussed.

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Selected References

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  1. Amadori M., Barei S., Melegari M., Panina G. F. Safety and efficacy of foot-and-mouth disease vaccines containing endonuclease-inactivated virions. Vaccine. 1987 Sep;5(3):219–222. doi: 10.1016/0264-410x(87)90104-6. [DOI] [PubMed] [Google Scholar]
  2. Andino R., Rieckhof G. E., Achacoso P. L., Baltimore D. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5'-end of viral RNA. EMBO J. 1993 Sep;12(9):3587–3598. doi: 10.1002/j.1460-2075.1993.tb06032.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BACHRACH H. L. Ribonucleic acid of foot-and-mouth disease virus: its preparation, stability, and plating efficiency on bovine-kidney cultures. Virology. 1960 Oct;12:258–271. doi: 10.1016/0042-6822(60)90199-9. [DOI] [PubMed] [Google Scholar]
  4. BROWN F., CARTWRIGHT B., STEWART D. L. The effect of various inactivating agents on the viral and ribonucleic acid infectivities of foot-and-mouth disease virus and on its attachment to susceptible cells. J Gen Microbiol. 1963 May;31:179–186. doi: 10.1099/00221287-31-2-179. [DOI] [PubMed] [Google Scholar]
  5. Brown F., Wild T. F. The effect of heat on the structure of foot-and-mouth disease virus and the viral ribonucleic acid. Biochim Biophys Acta. 1966 May 19;119(2):301–308. doi: 10.1016/0005-2787(66)90188-2. [DOI] [PubMed] [Google Scholar]
  6. Carrasco L. Picornavirus inhibitors. Pharmacol Ther. 1994;64(2):215–290. doi: 10.1016/0163-7258(94)90040-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cotmore S. F., Tattersall P. A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles. J Virol. 1989 Sep;63(9):3902–3911. doi: 10.1128/jvi.63.9.3902-3911.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Denoya C. D., Scodeller E. A., Vasquez C., La Torre J. L. Foot and mouth disease virus. II. Endoribonuclease activity within purified virions. Virology. 1978 Aug;89(1):67–74. doi: 10.1016/0042-6822(78)90040-5. [DOI] [PubMed] [Google Scholar]
  9. Diamond S. E., Kirkegaard K. Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis. J Virol. 1994 Feb;68(2):863–876. doi: 10.1128/jvi.68.2.863-876.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fenwick M. L. The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids. Biochem J. 1968 May;107(6):851–859. doi: 10.1042/bj1070851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gauntt C. J. Fragmentation of RNA in virus particles of rhinovirus type 14. J Virol. 1974 Mar;13(3):762–764. doi: 10.1128/jvi.13.3.762-764.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grigera P. R., Tisminetzky S. G., Lebendiker M. B., Periolo O. H., La Torre J. L. Presence of a 43-kDa host-cell polypeptide in purified aphthovirions. Virology. 1988 Aug;165(2):584–588. doi: 10.1016/0042-6822(88)90602-2. [DOI] [PubMed] [Google Scholar]
  13. Kolakofsky D., Altman S. Endoribonuclease activity associated with animal RNA viruses. J Virol. 1978 Jan;25(1):274–284. doi: 10.1128/jvi.25.1.274-284.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. La Torre J. L., Grubman M. J., Baxt B., Bachrach H. L. The structural polypeptides of aphthovirus are phosphoproteins. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7444–7447. doi: 10.1073/pnas.77.12.7444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Laporte J., Lenoir G. Structural proteins of foot-and-mouth disease virus. J Gen Virol. 1973 Aug;20(2):161–168. doi: 10.1099/0022-1317-20-2-161. [DOI] [PubMed] [Google Scholar]
  17. Lee W. M., Monroe S. S., Rueckert R. R. Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome. J Virol. 1993 Apr;67(4):2110–2122. doi: 10.1128/jvi.67.4.2110-2122.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Li J. P., Baltimore D. An intragenic revertant of a poliovirus 2C mutant has an uncoating defect. J Virol. 1990 Mar;64(3):1102–1107. doi: 10.1128/jvi.64.3.1102-1107.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Newman J. F., Piatti P. G., Gorman B. M., Burrage T. G., Ryan M. D., Flint M., Brown F. Foot-and-mouth disease virus particles contain replicase protein 3D. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):733–737. doi: 10.1073/pnas.91.2.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Newman J. F., Tirrell S., Ullman C., Piatti P. G., Brown F. Stabilising oral poliovaccine at high ambient temperatures. Vaccine. 1995;13(15):1431–1435. doi: 10.1016/0264-410x(95)00070-h. [DOI] [PubMed] [Google Scholar]
  21. Perlin M., Phillips B. A. In vitro assembly of polioviruses. 3. Assembly of 14 S particles into empty capsids by poliovirus-infected HeLa cell membranes. Virology. 1973 May;53(1):107–114. doi: 10.1016/0042-6822(73)90469-8. [DOI] [PubMed] [Google Scholar]
  22. Pfister T., Egger D., Bienz K. Poliovirus subviral particles associated with progeny RNA in the replication complex. J Gen Virol. 1995 Jan;76(Pt 1):63–71. doi: 10.1099/0022-1317-76-1-63. [DOI] [PubMed] [Google Scholar]
  23. Polatnick J. Effect of salts and other agents on foot-and-mouth disease virus poly (U) polymerase activity. Arch Virol. 1985;84(3-4):269–275. doi: 10.1007/BF01378979. [DOI] [PubMed] [Google Scholar]
  24. Sangar D. V., Rowlands D. J., Cavanagh D., Brown F. Characterization of the minor polypeptides in the foot-and-mouth disease particle. J Gen Virol. 1976 Apr;31(1):35–46. doi: 10.1099/0022-1317-31-1-35. [DOI] [PubMed] [Google Scholar]
  25. Scodeller E. A., Lebendiker M. A., Dubra M. S., Crespo O. A., Basarab O., La Torre J. L., Vasquez C. Inactivation of foot-and-mouth disease virus vaccine strains by activation of virus-associated endonuclease. J Gen Virol. 1984 Sep;65(Pt 9):1567–1573. doi: 10.1099/0022-1317-65-9-1567. [DOI] [PubMed] [Google Scholar]
  26. Stockley P. G., Stonehouse N. J., Valegård K. Molecular mechanism of RNA phage morphogenesis. Int J Biochem. 1994 Oct-Nov;26(10-11):1249–1260. doi: 10.1016/0020-711x(94)90094-9. [DOI] [PubMed] [Google Scholar]
  27. Ward R. L. Mechanism of poliovirus inactivation by ammonia. J Virol. 1978 May;26(2):299–305. doi: 10.1128/jvi.26.2.299-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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