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. 1997 Oct;71(10):7851–7856. doi: 10.1128/jvi.71.10.7851-7856.1997

Immunologic correlates of protection against rotavirus challenge after intramuscular immunization of mice.

S E Coffin 1, C A Moser 1, S Cohen 1, H F Clark 1, P A Offit 1
PMCID: PMC192140  PMID: 9311873

Abstract

The capacity of intramuscular (i.m.) inoculation of mice with homologous or heterologous host rotaviruses to induce protection from challenge was evaluated. i.m. inoculation with live, wild-type rotavirus (murine strain EDIM) induced complete protection from viral shedding after challenge for at least 6 weeks after inoculation; protection was correlated with production of virus-specific immunoglobulin A (IgA) by lamina propria (LP) lymphocytes. i.m. inoculation with inactivated EDIM, cell culture-adapted EDIM, or simian strain RRV was associated with partial protection, characterized by reduced viral shedding after challenge. Partial protection after challenge was not associated with production of virus-specific IgA by LP lymphocytes. The mechanisms by which i.m. inoculation induces virus-specific humoral immune responses in the small intestinal LP were examined.

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Selected References

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