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Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1992 Sep;36(9):1877–1882. doi: 10.1128/aac.36.9.1877

Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests.

K S Thomson 1, C C Sanders 1
PMCID: PMC192203  PMID: 1416878

Abstract

The three-dimensional and clavulanate double-disk potentiation tests were compared as procedures for the detection of extended-spectrum beta-lactamase production in 32 strains of Escherichia coli and Klebsiella pneumoniae, 31 of which produced TEM-1, TEM-2, TEM-3, TEM-4, TEM-5, TEM-7, TEM-8, TEM-9, TEM-10, TEM-12, TEM-101, SHV-1, SHV-2, SHV-3, SHV-4, SHV-5, CAZ-2, MIR-1, or an unidentified extended-spectrum beta-lactamase with a pI of 5.95, with some strains producing multiple beta-lactamases. The three-dimensional test, which was performed in conjunction with a routine disk diffusion test, detected extended-spectrum beta-lactamase production in 26 of 28 (93%) of the strains that produced extended-spectrum beta-lactamases. The clavulanate double-disk potentiation test detected extended-spectrum beta-lactamases in only 22 of the 28 strains (79%) when it was performed as currently recommended. The three-dimensional test, when performed in conjunction with the disk diffusion test, offered the advantages of providing simultaneous information about both antibiotic susceptibility and extended-spectrum beta-lactamase production, coupled with a greater sensitivity and earlier detection of extended-spectrum beta-lactamases.

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Selected References

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  1. Bauernfeind A., Chong Y., Schweighart S. Extended broad spectrum beta-lactamase in Klebsiella pneumoniae including resistance to cephamycins. Infection. 1989 Sep-Oct;17(5):316–321. doi: 10.1007/BF01650718. [DOI] [PubMed] [Google Scholar]
  2. Bauernfeind A., Grimm H., Schweighart S. A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infection. 1990 Sep-Oct;18(5):294–298. doi: 10.1007/BF01647010. [DOI] [PubMed] [Google Scholar]
  3. Bauernfeind A., Hörl G. Novel R-factor borne beta-lactamase of Escherichia coli confering resistance to cephalosporins. Infection. 1987 Jul-Aug;15(4):257–259. doi: 10.1007/BF01644127. [DOI] [PubMed] [Google Scholar]
  4. Bush K., Flamm R. K., Ohringer S., Singer S. B., Summerill R., Bonner D. P. Effect of clavulanic acid on activity of beta-lactam antibiotics in Serratia marcescens isolates producing both a TEM beta-lactamase and a chromosomal cephalosporinase. Antimicrob Agents Chemother. 1991 Nov;35(11):2203–2208. doi: 10.1128/aac.35.11.2203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casellas J. M., Goldberg M. Incidence of strains producing extended spectrum beta-lactamases in Argentina. Infection. 1989 Nov-Dec;17(6):434–436. doi: 10.1007/BF01645567. [DOI] [PubMed] [Google Scholar]
  6. Chanal C. M., Sirot D. L., Petit A., Labia R., Morand A., Sirot J. L., Cluzel R. A. Multiplicity of TEM-derived beta-lactamases from Klebsiella pneumoniae strains isolated at the same hospital and relationships between the responsible plasmids. Antimicrob Agents Chemother. 1989 Nov;33(11):1915–1920. doi: 10.1128/aac.33.11.1915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jacoby G. A., Archer G. L. New mechanisms of bacterial resistance to antimicrobial agents. N Engl J Med. 1991 Feb 28;324(9):601–612. doi: 10.1056/NEJM199102283240906. [DOI] [PubMed] [Google Scholar]
  8. Jacoby G. A., Carreras I. Activities of beta-lactam antibiotics against Escherichia coli strains producing extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1990 May;34(5):858–862. doi: 10.1128/aac.34.5.858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacoby G. A., Medeiros A. A. More extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1991 Sep;35(9):1697–1704. doi: 10.1128/aac.35.9.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jarlier V., Nicolas M. H., Fournier G., Philippon A. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988 Jul-Aug;10(4):867–878. doi: 10.1093/clinids/10.4.867. [DOI] [PubMed] [Google Scholar]
  11. Mabilat C., Courvalin P. Development of "oligotyping" for characterization and molecular epidemiology of TEM beta-lactamases in members of the family Enterobacteriaceae. Antimicrob Agents Chemother. 1990 Nov;34(11):2210–2216. doi: 10.1128/aac.34.11.2210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Papanicolaou G. A., Medeiros A. A., Jacoby G. A. Novel plasmid-mediated beta-lactamase (MIR-1) conferring resistance to oxyimino- and alpha-methoxy beta-lactams in clinical isolates of Klebsiella pneumoniae. Antimicrob Agents Chemother. 1990 Nov;34(11):2200–2209. doi: 10.1128/aac.34.11.2200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Petit A., Sirot D. L., Chanal C. M., Sirot J. L., Labia R., Gerbaud G., Cluzel R. A. Novel plasmid-mediated beta-lactamase in clinical isolates of Klebsiella pneumoniae more resistant to ceftazidime than to other broad-spectrum cephalosporins. Antimicrob Agents Chemother. 1988 May;32(5):626–630. doi: 10.1128/aac.32.5.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Philippon A., Ben Redjeb S., Fournier G., Ben Hassen A. Epidemiology of extended spectrum beta-lactamases. Infection. 1989 Sep-Oct;17(5):347–354. doi: 10.1007/BF01650727. [DOI] [PubMed] [Google Scholar]
  15. Philippon A., Labia R., Jacoby G. Extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1989 Aug;33(8):1131–1136. doi: 10.1128/aac.33.8.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rice L. B., Willey S. H., Papanicolaou G. A., Medeiros A. A., Eliopoulos G. M., Moellering R. C., Jr, Jacoby G. A. Outbreak of ceftazidime resistance caused by extended-spectrum beta-lactamases at a Massachusetts chronic-care facility. Antimicrob Agents Chemother. 1990 Nov;34(11):2193–2199. doi: 10.1128/aac.34.11.2193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rice L. B., Yao J. D., Klimm K., Eliopoulos G. M., Moellering R. C., Jr Efficacy of different beta-lactams against an extended-spectrum beta-lactamase-producing Klebsiella pneumoniae strain in the rat intra-abdominal abscess model. Antimicrob Agents Chemother. 1991 Jun;35(6):1243–1244. doi: 10.1128/aac.35.6.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sirot J., Chanal C., Petit A., Sirot D., Labia R., Gerbaud G. Klebsiella pneumoniae and other Enterobacteriaceae producing novel plasmid-mediated beta-lactamases markedly active against third-generation cephalosporins: epidemiologic studies. Rev Infect Dis. 1988 Jul-Aug;10(4):850–859. doi: 10.1093/clinids/10.4.850. [DOI] [PubMed] [Google Scholar]
  19. Thomson K. S., Mejglo Z. A., Pearce G. N., Regan T. J. 3-Dimensional susceptibility testing of beta-lactam antibiotics. J Antimicrob Chemother. 1984 Jan;13(1):45–54. doi: 10.1093/jac/13.1.45. [DOI] [PubMed] [Google Scholar]
  20. Thomson K. S., Sanders C. C., Washington J. A., 2nd High-level resistance to cefotaxime and ceftazidime in Klebsiella pneumoniae isolates from Cleveland, Ohio. Antimicrob Agents Chemother. 1991 May;35(5):1001–1003. doi: 10.1128/aac.35.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weber D. A., Sanders C. C., Bakken J. S., Quinn J. P. A novel chromosomal TEM derivative and alterations in outer membrane proteins together mediate selective ceftazidime resistance in Escherichia coli. J Infect Dis. 1990 Aug;162(2):460–465. doi: 10.1093/infdis/162.2.460. [DOI] [PubMed] [Google Scholar]
  22. Woodford N., Payne D. J., Johnson A. P., Weinbren M. J., Perinpanayagam R. M., George R. C., Cookson B. D., Amyes S. G. Transferable cephalosporin resistance not inhibited by clavulanate in Escherichia coli. Lancet. 1990 Jul 28;336(8709):253–253. doi: 10.1016/0140-6736(90)91784-8. [DOI] [PubMed] [Google Scholar]
  23. de Champs C., Sirot D., Chanal C., Poupart M. C., Dumas M. P., Sirot J. Concomitant dissemination of three extended-spectrum beta-lactamases among different Enterobacteriaceae isolated in a French hospital. J Antimicrob Chemother. 1991 Apr;27(4):441–457. doi: 10.1093/jac/27.4.441. [DOI] [PubMed] [Google Scholar]

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