Abstract
Recent improvements in electron microscope techniques which allow the study of virus fine structure have permitted the grouping of many viruses on a purely morphological basis. Briefly the techniques used in electron microscopy for the study of viruses are reviewed and the symmetry properties of virus particles as revealed by negative staining are discussed somewhat more fully.
Finally, virus particles are grouped on two bases, firstly the site of formation of the virus within the cell as seen by thin sectioning techniques, and secondly the symmetry property of the virus as seen by negative staining. Consideration of the groupings obtained in this way reveals that the biochemical and physical properties of a virus can be deduced from the readily established morphological characteristics.
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- ALMEIDA J. D., HOWATSON A. F. A negative staining method for cell-associated virus. J Cell Biol. 1963 Mar;16:616–620. doi: 10.1083/jcb.16.3.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
- CRICK F. H., WATSON J. D. Structure of small viruses. Nature. 1956 Mar 10;177(4506):473–475. doi: 10.1038/177473a0. [DOI] [PubMed] [Google Scholar]
- FRIEDMAN-KIEN A. E., ROWE W. P., BANFIELD W. G. Milker's nodules: isolation of a poxvirus from a human case. Science. 1963 Jun 21;140(3573):1335–1336. doi: 10.1126/science.140.3573.1335. [DOI] [PubMed] [Google Scholar]
- HAMPARIAN V. V., HILLEMAN M. R., KETLER A. Contributions to characterization and classification of animal viruses. Proc Soc Exp Biol Med. 1963 Apr;112:1040–1050. doi: 10.3181/00379727-112-28247. [DOI] [PubMed] [Google Scholar]
- HOWATSON A. F., WHITMORE G. F. The development and structure of vesicular stomatitis virus. Virology. 1962 Apr;16:466–478. doi: 10.1016/0042-6822(62)90228-3. [DOI] [PubMed] [Google Scholar]
- MCLEOD D. L., HAM A. W. SEARCH FOR ONCOGENIC PROPERTIES IN VARIOUS VIRUSES FOUND IN MAN: POSITIVE RESULTS WITH ADENOVIRUS TYPES 12 AND 18. Can Med Assoc J. 1963 Oct 19;89:799–805. [PMC free article] [PubMed] [Google Scholar]
- NAGINGTON J., HORNE R. W. Morphological studies of orf and vaccinia viruses. Virology. 1962 Mar;16:248–260. doi: 10.1016/0042-6822(62)90245-3. [DOI] [PubMed] [Google Scholar]
- NIXON H. L., WOODS R. D. The structure of tobacco mosaic virus protein. Virology. 1960 Jan;10:157–159. doi: 10.1016/0042-6822(60)90018-0. [DOI] [PubMed] [Google Scholar]