Tissue-specific modification of the human GCCR gene. DGG blot containing RsaI-digested DNA isolated from various tissues from single individuals, prepared from a 20–80% DGG, electrophoresed for 1500 VH, and hybridized with a 3.5 kb EcoRI GCCR gene fragment from the plasmid p24RI. Fragment 2 varies, depending upon the tissue source of DNA (doublets are seen in individual 406, fibroid, and individual 379 ovary). B = blood leukocyte, S = sperm, F = fibroid, Ov = ovary, Mu = muscle. Numbers indicate individual patients from whom tissues were collected.