Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1993 Feb;175(4):1081–1086. doi: 10.1128/jb.175.4.1081-1086.1993

Heterogeneity of small plasmids from halophilic archaea.

A S Akhmanova 1, V K Kagramanova 1, A S Mankin 1
PMCID: PMC193023  PMID: 8094388

Abstract

Small multicopy plasmids in three strains of halophilic archaea, SB3, GRB, and GN101, were found to be present in a cell as a population of related but not identical sequences. Two types of heterogeneity were observed: macroheterogeneity, represented by two major plasmid sequence versions homologous to each other by 80%, and microheterogeneity, in which individual plasmids differ by one or a few nucleotide substitutions.

Full text

PDF
1081

Images in this article

1082Image
on p.1082
1084Image
on p.1084
1085Image
on p.1085

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cline S. W., Doolittle W. F. Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons. J Bacteriol. 1992 Feb;174(3):1076–1080. doi: 10.1128/jb.174.3.1076-1080.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cline S. W., Lam W. L., Charlebois R. L., Schalkwyk L. C., Doolittle W. F. Transformation methods for halophilic archaebacteria. Can J Microbiol. 1989 Jan;35(1):148–152. doi: 10.1139/m89-022. [DOI] [PubMed] [Google Scholar]
  3. Couturier M., Bex F., Bergquist P. L., Maas W. K. Identification and classification of bacterial plasmids. Microbiol Rev. 1988 Sep;52(3):375–395. doi: 10.1128/mr.52.3.375-395.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DasSarma S., Damerval T., Jones J. G., Tandeau de Marsac N. A plasmid-encoded gas vesicle protein gene in a halophilic archaebacterium. Mol Microbiol. 1987 Nov;1(3):365–370. doi: 10.1111/j.1365-2958.1987.tb01943.x. [DOI] [PubMed] [Google Scholar]
  5. Hackett N. R., DasSarma S. Characterization of the small endogenous plasmid of Halobacterium strain SB3 and its use in transformation of H. halobium. Can J Microbiol. 1989 Jan;35(1):86–91. doi: 10.1139/m89-013. [DOI] [PubMed] [Google Scholar]
  6. Hackett N. R., Krebs M. P., DasSarma S., Goebel W., RajBhandary U. L., Khorana H. G. Nucleotide sequence of a high copy number plasmid from Halobacterium strain GRB. Nucleic Acids Res. 1990 Jun 11;18(11):3408–3408. doi: 10.1093/nar/18.11.3408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hall M. J., Hackett N. R. DNA sequence of a small plasmid from Halobacterium strain GN101. Nucleic Acids Res. 1989 Dec 25;17(24):10501–10501. doi: 10.1093/nar/17.24.10501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  9. Holmes M. L., Dyall-Smith M. L. A plasmid vector with a selectable marker for halophilic archaebacteria. J Bacteriol. 1990 Feb;172(2):756–761. doi: 10.1128/jb.172.2.756-761.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ilyina T. V., Koonin E. V. Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria. Nucleic Acids Res. 1992 Jul 11;20(13):3279–3285. doi: 10.1093/nar/20.13.3279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kagramanova V. K., Derckacheva N. I., Mankin A. S. The complete nucleotide sequence of the arcaebacterial plasmid pHSB from Halobacterium, strain SB3. Nucleic Acids Res. 1988 May 11;16(9):4158–4158. doi: 10.1093/nar/16.9.4158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kagramanova V. K., Derckacheva N. I., Mankin A. S. Unusual nucleotide sequence heterogeneity of small multicopy pHSB plasmid from Halobacterium strain SB3, an archaebacterium. Can J Microbiol. 1989 Jan;35(1):160–163. doi: 10.1139/m89-024. [DOI] [PubMed] [Google Scholar]
  13. Krebs M. P., Hauss T., Heyn M. P., RajBhandary U. L., Khorana H. G. Expression of the bacterioopsin gene in Halobacterium halobium using a multicopy plasmid. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):859–863. doi: 10.1073/pnas.88.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mount D. W., Conrad B. Improved programs for DNA and protein sequence analysis on the IBM personal computer and other standard computer systems. Nucleic Acids Res. 1986 Jan 10;14(1):443–454. doi: 10.1093/nar/14.1.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Novick R. P. Plasmid incompatibility. Microbiol Rev. 1987 Dec;51(4):381–395. doi: 10.1128/mr.51.4.381-395.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pfeifer F., Blaseio U., Ghahraman P. Dynamic plasmid populations in Halobacterium halobium. J Bacteriol. 1988 Aug;170(8):3718–3724. doi: 10.1128/jb.170.8.3718-3724.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sioud M., Baldacci G., Forterre P., de Recondo A. M. Novobiocin induces accumulation of a single strand of plasmid pGRB-1 in the archaebacterium Halobacterium GRB. Nucleic Acids Res. 1988 Aug 25;16(16):7833–7842. doi: 10.1093/nar/16.16.7833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woese C. R., Kandler O., Wheelis M. L. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4576–4579. doi: 10.1073/pnas.87.12.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES