Figure 1.—

Strategy for producing F₂ lines of M. hapla. A culture with young females (F₀) of strain VW8 (which lacks PCR marker H1) is inoculated with males (curved line) of strain VW9 (which carries marker H1). After 2 weeks, egg masses are collected from VW8 F₀ females and tested for the presence of marker H1. Juveniles from egg masses with marker H1 are inoculated onto plants and allowed to develop parthenogenetically into F₁ females. Egg masses from F₁ females are tested for the presence of marker H1. Eggs from F₂ egg masses are inoculated onto individual plants. This figure is adapted from Williamson and Liu (2006).