Abstract
The hemagglutinin of all three types of reovirus was inactivated by chloroform with little or no diminution in virus infectivity. Albumin and other proteins protected the hemagglutinin from inactivation by chloroform. When crude reovirus suspensions were centrifuged in a sucrose density gradient, two hemagglutinin fractions were collected. The fraction which sedimented more rapidly contained the bulk of infectious virus, but both lost hemagglutinin activity when extracted with chloroform. Ether extraction of the fractions did not reduce either the infectivity or hemagglutinin titre.
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Selected References
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- FELDMAN H. A., WANG S. S. Sensitivity of various viruses to chloroform. Proc Soc Exp Biol Med. 1961 Apr;106:736–738. doi: 10.3181/00379727-106-26459. [DOI] [PubMed] [Google Scholar]
- GOMATOS P. J., TAMM I., DALES S., FRANKLIN R. M. Reovirus type 3: physical characteristics and interaction with L cells. Virology. 1962 Jul;17:441–454. doi: 10.1016/0042-6822(62)90139-3. [DOI] [PubMed] [Google Scholar]
- Leers W. D., Rozee K. R. A survey of reovirus antibodies in sera of urban children. Can Med Assoc J. 1966 May 14;94(20):1040–1042. [PMC free article] [PubMed] [Google Scholar]
- ROSEN L., HOVIS J. F., MASTROTA F. M., BELL J. A., HUEBNER R. J. Observations on a newly recognized virus (Abney) of the reovirus family. Am J Hyg. 1960 Mar;71:258–265. doi: 10.1093/oxfordjournals.aje.a120109. [DOI] [PubMed] [Google Scholar]
- SABIN A. B. Reoviruses. A new group of respiratory and enteric viruses formerly classified as ECHO type 10 is described. Science. 1959 Nov 20;130(3386):1387–1389. doi: 10.1126/science.130.3386.1387. [DOI] [PubMed] [Google Scholar]