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. 1972 Feb 19;106(4):327–330.

The significance of specific IgM antibody in the diagnosis of rubella employing the immunofluorescence technique

S Iwakata, A J Rhodes, N A Labzoffsky
PMCID: PMC1940404  PMID: 4551395

Abstract

The applicability of the immunofluorescence (IF) test to the diagnosis of primary rubella infection was investigated. The test is based on the detection of rubella-specific antibodies in the IgM fraction of immunoglobulins. The results indicate the usefulness of the IF test for the diagnosis of primary rubella infection on a single serum specimen collected at a proper time. The test is also of value in the differentiation of primary infection from reinfection, since in reinfection no rubella-specific antibodies are found in the IgM fraction. The test is also valuable for the detection of fetal infection in utero since the persistence of IgM antibodies in pregnant women is indicative of fetal infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banatvala J. E., Best J. M., Kennedy E. A., Smith E. E., Spence M. E. A serological method for demonstrating recent infection by rubella virus. Br Med J. 1967 Jul 29;3(5560):285–286. doi: 10.1136/bmj.3.5560.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baublis J. V., Brown G. C. Specific response of the immunoglobulins to rubella infection. Proc Soc Exp Biol Med. 1968 May;128(1):206–210. doi: 10.3181/00379727-128-32979. [DOI] [PubMed] [Google Scholar]
  3. Best J. M., Banatvala J. E., Watson D. Serum IgM and IgG responses in postnatally acquired rubella. Lancet. 1969 Jul 12;2(7611):65–68. doi: 10.1016/s0140-6736(69)92386-1. [DOI] [PubMed] [Google Scholar]
  4. Brown G. C., Baublis J. V., O'Leary T. P. Development and duration of mumps fluorescent antibodies in various immunoglobulin fractions of human serum. J Immunol. 1970 Jan;104(1):86–94. [PubMed] [Google Scholar]
  5. Brown G. C., O'leary T. P. Fluorescent-antibody marker for vaccine-induced rubella antibodies. Infect Immun. 1970 Oct;2(4):360–363. doi: 10.1128/iai.2.4.360-363.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S. M., Ducharme C. P., Carpenter C. A., Deibel R. Rubella antibody in IgG and IgM immunoglobulins detected by immunofluorescence. J Lab Clin Med. 1968 Nov;72(5):760–766. [PubMed] [Google Scholar]
  7. Cooper L. Z., Matters B., Rosenblum J. K., Krugman S. Experience with a modified rubella hemagglutination inhibition antibody test. JAMA. 1969 Jan 6;207(1):89–93. [PubMed] [Google Scholar]
  8. Haire M., Hadden D. S. Immunoglobulin responses in rubella and its complications. Br Med J. 1970 Jul 18;3(5715):130–132. doi: 10.1136/bmj.3.5715.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Langenhuysen M. M., The T. H., Nieweg H. O., Kapsenberg J. G. Demonstration of IgM cytomegalovirus-antibodies as an aid to early diagnosis in adults. Clin Exp Immunol. 1970 Mar;6(3):387–393. [PMC free article] [PubMed] [Google Scholar]
  10. Lennette E. H., Schmidt N. J. Rubella. Technical problems in the performance of hemagglutination-inhibition (HI) tests. Calif Med. 1969 Nov;111(5):351–354. [PMC free article] [PubMed] [Google Scholar]
  11. Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]
  12. UHR J. W., FINKELSTEIN M. S. Antibody formation. IV. Formation of rapidly and slowly sedimenting antibodies and immunological memory to bacteriophage phi-X 174. J Exp Med. 1963 Mar 1;117:457–477. doi: 10.1084/jem.117.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]

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