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(A) Genomic sequence and targeting constructs with the resultant NRF2 knockout gene. (B) Southern blot analysis of ES cells. An external probe XbaI–HindIII fragment gave a 15.5-kb band for the wild-type and 16.5-kb band for the homologous recombinant. (C) Southern blot analysis of F2 offspring. A StuI–EcoRV probe gave a 2.4-kb band for the wild type and a 1.5-kb band for the recombinant.
