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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Methods. 2007 Jul;42(3):278–288. doi: 10.1016/j.ymeth.2007.02.005

Fig. 4.

Fig. 4

(A) Selective inhibition of I219A PTP1B by compound 2a. Compound 2a (25 μM) was incubated at 22 °C with 100 mM NaOAc pH 5.2; 50 mM NaCl; pNPP (concentration corresponding to the Km value for the particular enzyme); and the indicated PTP. Percent PTP1B activities in the presence of 2a (normalized to a no-inhibitor control) are shown as bars. (B) Selective inhibition of I219A PTP1B by compound 3.Compound 3 (20 μM) was incubated at 22 °C with 50 mM 3,3-dimethylglutarate pH 7.0; 1 mM EDTA; 50 mM NaCl; pNPP (concentration corresponding to the KM for the particular enzyme); and the indicated PTP. Percent PTP1B activities in the presence of 3 (normalized to a no-inhibitor control) are shown as bars.