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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Methods. 2007 Jul;42(3):278–288. doi: 10.1016/j.ymeth.2007.02.005

Table 2.

Kinetic constants for inhibitor-sensitized HePTP mutants, assayed with pNPP as substrate at pH 7.0

PTP kcat (s−1) KM (mM) kcat/KM (s−1 mM−1)
wild-type HePTP 1.3 ± 0.39 6.9 ± 3.0 0.19
I107A HePTP 1.7 ± 0.49 6.2 ± 2.4 0.27
I274A HePTP 1.3 ± 0.33 7.3 ± 2.7 0.18