Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 Jan;58(1):119–124. doi: 10.1128/aem.58.1.119-124.1992

Differential Siderophore Utilization and Iron Uptake by Soil and Rhizosphere Bacteria

Edouard Jurkevitch 1, Yitzhak Hadar 1,*, Yona Chen 1
PMCID: PMC195181  PMID: 16348618

Abstract

The differential availabilities of the hydroxamate siderophores ferrioxamine B (FOB) and ferrichrome (FC) and the pseudobactin siderophores St3, 7NSK2, and WCS 358 as sources of Fe for soil and rhizosphere bacteria were studied. About 20% of the total bacterial CFU from the rhizospheres of four plant species were able to use FOB as the sole Fe source in an Fe-deficient medium, while about 12, 10, 2, and > 1% were able to use FC and pseudobactins 7NSK2, St3, and WCS 358, respectively. Of the 165 colonies isolated from plates containing pseudobactins, 64 were able to use the pseudobactin on which they were isolated as the sole Fe source in pure culture. Cross-feeding tests showed that almost all of these 64 strains were also able to use at least one of the other siderophores studied (pseudobactin, FOB, or FC). Pseudomonas putida StS2, Pseudomonas maltophilia 7NM1, and Vibrio fluvialis WS1, which were originally isolated on pseudobactins St3, 7NSK2, and WCS 358, respectively, were selected for their ability to grow with pseudobactin St3 as the sole Fe source. They incorporated 55Fe3+ mediated by pseudobactin St3 at various rates (71.5, 4, and 23 pmol/min/mg [dry weight] of cells, respectively). Similarly, P. putida St3 was shown to incorporate 55Fe3+ mediated by FOB and FC. We suggest that the ability of bacteria to utilize a large variety of siderophores confers an ecological advantage.

Full text

PDF
119

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buyer J. S., Leong J. Iron transport-mediated antagonism between plant growth-promoting and plant-deleterious Pseudomonas strains. J Biol Chem. 1986 Jan 15;261(2):791–794. [PubMed] [Google Scholar]
  2. Crowley D. E., Reid C. P., Szaniszlo P. J. Utilization of microbial siderophores in iron acquisition by oat. Plant Physiol. 1988 Jul;87(3):680–685. doi: 10.1104/pp.87.3.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hohnadel D., Meyer J. M. Specificity of pyoverdine-mediated iron uptake among fluorescent Pseudomonas strains. J Bacteriol. 1988 Oct;170(10):4865–4873. doi: 10.1128/jb.170.10.4865-4873.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Konetschny-Rapp S., Huschka H. G., Winkelmann G., Jung G. High-performance liquid chromatography of siderophores from fungi. Biol Met. 1988;1(1):9–17. doi: 10.1007/BF01128012. [DOI] [PubMed] [Google Scholar]
  5. Lesuisse E., Labbe P. Reductive and non-reductive mechanisms of iron assimilation by the yeast Saccharomyces cerevisiae. J Gen Microbiol. 1989 Feb;135(2):257–263. doi: 10.1099/00221287-135-2-257. [DOI] [PubMed] [Google Scholar]
  6. Liu P. V., Shokrani F. Biological activities of pyochelins: iron-chelating agents of Pseudomonas aeruginosa. Infect Immun. 1978 Dec;22(3):878–890. doi: 10.1128/iai.22.3.878-890.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moody M. D., Dailey H. A. Siderophore utilization and iron uptake by Rhodopseudomonas sphaeroides. Arch Biochem Biophys. 1984 Oct;234(1):178–186. doi: 10.1016/0003-9861(84)90339-4. [DOI] [PubMed] [Google Scholar]
  8. Müller G., Matzanke B. F., Raymond K. N. Iron transport in Streptomyces pilosus mediated by ferrichrome siderophores, rhodotorulic acid, and enantio-rhodotorulic acid. J Bacteriol. 1984 Oct;160(1):313–318. doi: 10.1128/jb.160.1.313-318.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  10. Poole K., Young L., Neshat S. Enterobactin-mediated iron transport in Pseudomonas aeruginosa. J Bacteriol. 1990 Dec;172(12):6991–6996. doi: 10.1128/jb.172.12.6991-6996.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rogers H. J. Iron-Binding Catechols and Virulence in Escherichia coli. Infect Immun. 1973 Mar;7(3):445–456. doi: 10.1128/iai.7.3.445-456.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Royt P. W. Isolation of a membrane associated iron chelator from Pseudomonas aeruginosa. Biochim Biophys Acta. 1988 Apr 22;939(3):493–502. doi: 10.1016/0005-2736(88)90096-x. [DOI] [PubMed] [Google Scholar]
  13. Schwyn B., Neilands J. B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987 Jan;160(1):47–56. doi: 10.1016/0003-2697(87)90612-9. [DOI] [PubMed] [Google Scholar]
  14. Zimmermann L., Angerer A., Braun V. Mechanistically novel iron(III) transport system in Serratia marcescens. J Bacteriol. 1989 Jan;171(1):238–243. doi: 10.1128/jb.171.1.238-243.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. de Weger L. A., van Arendonk J. J., Recourt K., van der Hofstad G. A., Weisbeek P. J., Lugtenberg B. Siderophore-mediated uptake of Fe3+ by the plant growth-stimulating Pseudomonas putida strain WCS358 and by other rhizosphere microorganisms. J Bacteriol. 1988 Oct;170(10):4693–4698. doi: 10.1128/jb.170.10.4693-4698.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES