Abstract
Enterotoxin-positive (Ent+) and enterotoxin-negative (Ent-) strains of Clostridium perfringens were cultured in Duncan-Strong sporulation medium containing starch at 37 and 46 degrees C. At 37 degrees C, all strains degraded starch and sporulated well. However, only Ent- strains could hydrolyze starch, grow extensively, and sporulate at 46 degrees C. Growth, sporulation, and starch hydrolysis by Ent+ strains at 46 degrees C were equivalent to those obtained at 37 degrees C when alpha-amylase was added to the cultures during growth. The total amount of extracellular plus intracellular amylase in cultures of Ent+ strains was significantly less in cells incubated at 46 degrees C than in cells incubated at 37 degrees C. These results contradict an earlier report that Ent+ strains cannot sporulate well near their optimal growth temperature (R. G. Labbe and C. L. Duncan, Can. J. Microbiol. 20:1493-1501, 1974) and suggest that synthesis of alpha-amylase in Ent+ strains is regulated by temperature.
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- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Duncan C. L., Strong D. H. Improved medium for sporulation of Clostridium perfringens. Appl Microbiol. 1968 Jan;16(1):82–89. doi: 10.1128/am.16.1.82-89.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELLNER P. D. A medium promoting rapid quantitative sporulation in Clostridium perfringens. J Bacteriol. 1956 Apr;71(4):495–496. doi: 10.1128/jb.71.4.495-496.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hao J., Pazlarová J., Strnadová M., Chaloupka J. Regulation of extracellular proteins and alpha-amylase secretion by temperature in Bacillus subtilis. Folia Microbiol (Praha) 1989;34(3):179–184. doi: 10.1007/BF02821290. [DOI] [PubMed] [Google Scholar]
- Kim C. H., Cheney R., Woodburn M. Sporulation of Clostridium perfringens in a modified medium and selected foods. Appl Microbiol. 1967 Jul;15(4):871–876. doi: 10.1128/am.15.4.871-876.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labbe R. G., Duncan C. L. Influence of carbohydrates on growth and sporulation of Clostridium perfringens type A. Appl Microbiol. 1975 Mar;29(3):345–351. doi: 10.1128/am.29.3.345-351.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labbe R. G., Duncan C. L. Sporulation and enterotoxin production by Clostridium perfringens type A under conditions of controlled pH and temperature. Can J Microbiol. 1974 Nov;20(11):1493–1501. doi: 10.1139/m74-233. [DOI] [PubMed] [Google Scholar]
- Labbe R., Somers E., Duncan C. Influence of starch source on sporulation and enterotoxin production by Clostridium perfringens type A. Appl Environ Microbiol. 1976 Mar;31(3):455–457. doi: 10.1128/aem.31.3.455-457.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skjelkvåle R., Uemura T. Experimental Diarrhoea in human volunteers following oral administration of Clostridium perfringens enterotoxin. J Appl Bacteriol. 1977 Oct;43(2):281–286. doi: 10.1111/j.1365-2672.1977.tb00752.x. [DOI] [PubMed] [Google Scholar]
- Strong D. H., Duncan C. L., Perna G. Clostridium perfringens Type A Food Poisoning II. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Human Beings. Infect Immun. 1971 Jan;3(1):171–178. doi: 10.1128/iai.3.1.171-178.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tórtora J. C. Alternative medium for Clostridium perfringens sporulation. Appl Environ Microbiol. 1984 May;47(5):1172–1174. doi: 10.1128/aem.47.5.1172-1174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]