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. 1992 Jan;58(1):399–402. doi: 10.1128/aem.58.1.399-402.1992

Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50.

A C Layton 1, J Sanseverino 1, W Wallace 1, C Corcoran 1, G S Sayler 1
PMCID: PMC195221  PMID: 1539985

Abstract

The biodegradation of 4-chlorobiphenyl usually proceeds through the intermediate 4-chlorobenzoate. Few bacterial strains can degrade 4-chlorobiphenyl to 4-chlorobenzoate and 4-chlorobenzoate to CO2. This study demonstrates that the 4-chlorobiphenyl-degrading Alcaligenes sp. strain ALP83 can degrade 4-chlorobenzoate to 4-hydroxybenzoate. The dehalogenase activity is correlated with a 10-kb fragment carried on plasmid pSS70.

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Selected References

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  1. Adriaens P., Kohler H. P., Kohler-Staub D., Focht D. D. Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4'-dichlorobiphenyl. Appl Environ Microbiol. 1989 Apr;55(4):887–892. doi: 10.1128/aem.55.4.887-892.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burlage R. S., Bemis L. A., Layton A. C., Sayler G. S., Larimer F. Comparative genetic organization of incompatibility group P degradative plasmids. J Bacteriol. 1990 Dec;172(12):6818–6825. doi: 10.1128/jb.172.12.6818-6825.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elsner A., Löffler F., Miyashita K., Müller R., Lingens F. Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components. Appl Environ Microbiol. 1991 Jan;57(1):324–326. doi: 10.1128/aem.57.1.324-326.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Furukawa K., Chakrabarty A. M. Involvement of plasmids in total degradation of chlorinated biphenyls. Appl Environ Microbiol. 1982 Sep;44(3):619–626. doi: 10.1128/aem.44.3.619-626.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  6. Hooper S. W., Dockendorff T. C., Sayler G. S. Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50. Appl Environ Microbiol. 1989 May;55(5):1286–1288. doi: 10.1128/aem.55.5.1286-1288.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marks T. S., Smith A. R., Quirk A. V. Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp. Appl Environ Microbiol. 1984 Nov;48(5):1020–1025. doi: 10.1128/aem.48.5.1020-1025.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Massé R., Messier F., Péloquin L., Ayotte C., Sylvestre M. Microbial biodegradation of 4-chlorobiphenyl, a model compound of chlorinated biphenyls. Appl Environ Microbiol. 1984 May;47(5):947–951. doi: 10.1128/aem.47.5.947-951.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pettigrew C. A., Breen A., Corcoran C., Sayler G. S. Chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria. Appl Environ Microbiol. 1990 Jul;56(7):2036–2045. doi: 10.1128/aem.56.7.2036-2045.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reineke W., Knackmuss H. J. Microbial degradation of haloaromatics. Annu Rev Microbiol. 1988;42:263–287. doi: 10.1146/annurev.mi.42.100188.001403. [DOI] [PubMed] [Google Scholar]
  11. Savard P., Péloquin L., Sylvestre M. Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate. J Bacteriol. 1986 Oct;168(1):81–85. doi: 10.1128/jb.168.1.81-85.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sayler G. S., Lund L. C., Shiaris M. P., Sherrill T. W., Perkins R. E. Comparative effects of Aroclor 1254 (polychlorinated biphenyls) and phenanthrene on glucose uptake by freshwater microbial populations. Appl Environ Microbiol. 1979 May;37(5):878–885. doi: 10.1128/aem.37.5.878-885.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scholten J. D., Chang K. H., Babbitt P. C., Charest H., Sylvestre M., Dunaway-Mariano D. Novel enzymic hydrolytic dehalogenation of a chlorinated aromatic. Science. 1991 Jul 12;253(5016):182–185. doi: 10.1126/science.1853203. [DOI] [PubMed] [Google Scholar]
  14. Shields M. S., Hooper S. W., Sayler G. S. Plasmid-mediated mineralization of 4-chlorobiphenyl. J Bacteriol. 1985 Sep;163(3):882–889. doi: 10.1128/jb.163.3.882-889.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zervos P. H., Morris L. M., Hellwig R. J. A novel method for rapid isolation of plasmid DNA. Biotechniques. 1988 Mar;6(3):238–242. [PubMed] [Google Scholar]
  16. van den Tweel W. J., Kok J. B., de Bont J. A. Reductive dechlorination of 2,4-dichlorobenzoate to 4-chlorobenzoate and hydrolytic dehalogenation of 4-chloro-, 4-bromo-, and 4-iodobenzoate by Alcaligenes denitrificans NTB-1. Appl Environ Microbiol. 1987 Apr;53(4):810–815. doi: 10.1128/aem.53.4.810-815.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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