Table 2a.

Steroid levels by MS/MS after ACTH stimulation in normal subjects (males and females combined)

	Steroid leve l0 mins	Steroid level 30 mins	Steroid level 60 mins	Steroid Peak (maximum response to ACTH stimulation)	Increase Significant (0 mins to Peak)
Cortisol (μg/dL)
combined	11.50 ± 5.38	26.57 ± 6.17	31.07 ± 7.85	31.13 ± 7.75	p < 0.001
Aldosterone (ng/dL)
combined	4.57 ± 3.56	12.04 ± 7.25	9.53 ± 5.58	12.52 ± 7.12	p < 0.001
11-deoxycortisol (ng/dL)
combined	79 ± 113	571 ± 263	670 ± 290	676 ± 286	p < 0.001
DHEA (ng/dL)
normal	329 ± 399	727 ± 410	810 ± 420	823 ± 416	p < 0.001
normal standard of care	145 ± 196	376 ± 333	419 ± 367	432 ± 366
combined	209 ± 296	496 ± 398	553 ± 429	567 ± 427
17-OH progesterone (ng/dL)
combined	47 ± 53	150 ± 142	162 ± 163	167 ± 162	p < 0.001
Androstenedione (ng/mL)
combined	0.43 ± 0.41	0.75 ± 0.50	0.78 ± 0.50	0.82 ± 0.50	p < 0.001
DHEA-S (μg/dL)
normal	248 ± 180	262 ± 183	273 ± 180	281 ± 188	p < 0.001
normal standard of care	67 ± 66	62 ± 59	72 ± 67	75 ± 68
combined	129 ± 146	131 ± 151	141 ± 152	146 ± 157