Abstract
The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10-5 to 10-7 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidans at a frequency of 1.0 × 10-3 per recipient.
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- Bennett P. M., Grinsted J., Richmond M. H. Transposition of TnA does not generate deletions. Mol Gen Genet. 1977 Jul 20;154(2):205–211. doi: 10.1007/BF00330839. [DOI] [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson M. S., Summers A. O. Wide-host-range plasmids function in the genus thiobacillus. Appl Environ Microbiol. 1983 Sep;46(3):565–572. doi: 10.1128/aem.46.3.565-572.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
- Kulpa C. F., Roskey M. T., Travis M. T. Transfer of plasmid RP1 into chemolithotrophic Thiobacillus neapolitanus. J Bacteriol. 1983 Oct;156(1):434–436. doi: 10.1128/jb.156.1.434-436.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plasota M., Piechucka E., Kauc B., Wlodarczyk M. R68.45 plasmid mediated conjugation in Thiobacillus A2. Microbios. 1984;41(164):81–89. [PubMed] [Google Scholar]
- Yan W. [Cloning and expression of a promoter function fragment from Thiobacillus thiooxidans in Escherichia coli]. Yi Chuan Xue Bao. 1990;17(2):143–147. [PubMed] [Google Scholar]