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. 1992 Feb;58(2):727–730. doi: 10.1128/aem.58.2.727-730.1992

Preparation of Encapsulated Microbial Cells for Environmental Applications

Keith E Stormo 1, Ronald L Crawford 1,*
PMCID: PMC195313  PMID: 16348656

Abstract

An improved method for the encapsulation of bacteria into microspheres of alginate, agarose, or polyurethane is described. Cell suspensions were passed through a low-pressure nozzle into an aqueous phase where matrix polymerization or gelation yielded beads 2 to 50 μm in diameter. Trials with a chlorophenol-degrading Flavobacterium species showed that cells entrapped by these procedures were as catabolically active as free cells. These types of beads should have numerous applications in the fields of environmental science and engineering.

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Selected References

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  1. Fukushima Y., Okamura K., Imai K., Motai H. A new immobilization technique of whole cells and enzymes with colloidal silica and alginate. Biotechnol Bioeng. 1988 Aug 20;32(5):584–594. doi: 10.1002/bit.260320503. [DOI] [PubMed] [Google Scholar]
  2. Hulst A. C., Tramper J., Van't Riet K., Westerbeek J. M. A new technique for the production of immobilized biocatalyst in large quantities. Biotechnol Bioeng. 1985 Jun;27(6):870–876. doi: 10.1002/bit.260270617. [DOI] [PubMed] [Google Scholar]
  3. Jackson D. A., Cook P. R. A cell-cycle-dependent DNA polymerase activity that replicates intact DNA in chromatin. J Mol Biol. 1986 Nov 5;192(1):65–76. doi: 10.1016/0022-2836(86)90464-x. [DOI] [PubMed] [Google Scholar]
  4. Jackson D. A., Cook P. R. Replication occurs at a nucleoskeleton. EMBO J. 1986 Jun;5(6):1403–1410. doi: 10.1002/j.1460-2075.1986.tb04374.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nilsson H., Mosbach R., Mosbach K. The use of bead polymerization of acrylic monomers for immobilization of enzymes. Biochim Biophys Acta. 1972 Apr 7;268(1):253–256. doi: 10.1016/0005-2744(72)90222-7. [DOI] [PubMed] [Google Scholar]
  6. Nilsson K., Scheirer W., Merten O. W., Ostberg L., Liehl E., Katinger H. W., Mosbach K. Entrapment of animal cells for production of monoclonal antibodies and other biomolecules. Nature. 1983 Apr 14;302(5909):629–630. doi: 10.1038/302629a0. [DOI] [PubMed] [Google Scholar]
  7. O'Reilly K. T., Crawford R. L. Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells. Appl Environ Microbiol. 1989 Sep;55(9):2113–2118. doi: 10.1128/aem.55.9.2113-2118.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pras N., Hesselink P. G., ten Tusscher J., Malingré T. M. Kinetic aspects of the bioconversion of L-tyrosine into L-DOPA by cells of Mucuna pruriensL. Entrapped in different matrices. Biotechnol Bioeng. 1989 Jun 20;34(2):214–222. doi: 10.1002/bit.260340210. [DOI] [PubMed] [Google Scholar]
  9. Sun Y., Furusaki S., Yamauchi A., Ichimura K. Diffusivity of oxygen into carriers entrapping whole cells. Biotechnol Bioeng. 1989 Jun 5;34(1):55–58. doi: 10.1002/bit.260340107. [DOI] [PubMed] [Google Scholar]
  10. Topp E., Crawford R. L., Hanson R. S. Influence of readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp. Appl Environ Microbiol. 1988 Oct;54(10):2452–2459. doi: 10.1128/aem.54.10.2452-2459.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Topp E., Hanson R. S. Degradation of pentachlorophenol by a Flavobacterium species grown in continuous culture under various nutrient limitations. Appl Environ Microbiol. 1990 Feb;56(2):541–544. doi: 10.1128/aem.56.2.541-544.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vetvicka V., Fornůsek L. Polymer microbeads in immunology. Biomaterials. 1987 Sep;8(5):341–345. doi: 10.1016/0142-9612(87)90003-2. [DOI] [PubMed] [Google Scholar]
  13. Yang C. P., Su C. S. Synthesis of aspartame precursor: alpha-L-aspartyl-L-phenylalanine methyl ester in ethyl acetate using thermolysin entrapped in polyurethane. Biotechnol Bioeng. 1988 Aug 20;32(5):595–603. doi: 10.1002/bit.260320504. [DOI] [PubMed] [Google Scholar]

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