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. 1992 Feb;58(2):754–757. doi: 10.1128/aem.58.2.754-757.1992

Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.

Y L Tsai 1, B H Olson 1
PMCID: PMC195321  PMID: 1610201

Abstract

Polymerase chain reaction was used to amplify the low copy number of two 16S ribosomal gene fragments from soil and sediment extracts. Total DNA for polymerase chain reaction was extracted from 1 g of seeded or unseeded samples by a rapid freeze-and-thaw method. Amplified DNA fragments can be detected in DNA fractions isolated from seeded soil containing less than 3 Escherichia coli cells and from seeded sediments containing less than 10 cells. This research demonstrated that coupling polymerase chain reaction to direct DNA extraction improves sensitivity by 1 and 2 orders of magnitude for sediments and soils, respectively. This technique could become a powerful tool for genetic ecology studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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