Abstract
Toluene and the three isomers of xylene were completely mineralized to CO2 and biomass by aquifer-derived microorganisms under strictly anaerobic conditions. The source of the inoculum was gasoline-contaminated sediment from Seal Beach, Calif. Evidence confirming that sulfate was the terminal electron acceptor is presented. Benzene and ethylbenzene were not degraded under the experimental conditions used. Successive transfers of the mixed cultures that were enriched from aquifer sediments retained the ability to degrade toluene and xylenes. Greater than 90% of 14C-labeled toluene or 14C-labeled o-xylene was mineralized to 14CO2. The doubling time for the culture grown on toluene or m-xylene was about 20 days, and the cell yield was about 0.1 to 0.14 g of cells (dry weight) per g of substrate. The accumulation of sulfide in the cultures as a result of sulfate reduction appeared to inhibit degradation of aromatic hydrocarbons.
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Selected References
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- Beller H. R., Grbić-Galić D., Reinhard M. Microbial degradation of toluene under sulfate-reducing conditions and the influence of iron on the process. Appl Environ Microbiol. 1992 Mar;58(3):786–793. doi: 10.1128/aem.58.3.786-793.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean B. J. Recent findings on the genetic toxicology of benzene, toluene, xylenes and phenols. Mutat Res. 1985 Nov;154(3):153–181. doi: 10.1016/0165-1110(85)90016-8. [DOI] [PubMed] [Google Scholar]
- Dolfing J., Zeyer J., Binder-Eicher P., Schwarzenbach R. P. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch Microbiol. 1990;154(4):336–341. doi: 10.1007/BF00276528. [DOI] [PubMed] [Google Scholar]
- Evans P. J., Mang D. T., Young L. Y. Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures. Appl Environ Microbiol. 1991 Feb;57(2):450–454. doi: 10.1128/aem.57.2.450-454.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grbić-Galić D., Vogel T. M. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. doi: 10.1128/aem.53.2.254-260.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gunsalus R. P., Romesser J. A., Wolfe R. S. Preparation of coenzyme M analogues and their activity in the methyl coenzyme M reductase system of Methanobacterium thermoautotrophicum. Biochemistry. 1978 Jun 13;17(12):2374–2377. doi: 10.1021/bi00605a019. [DOI] [PubMed] [Google Scholar]
- Kuhn E. P., Zeyer J., Eicher P., Schwarzenbach R. P. Anaerobic degradation of alkylated benzenes in denitrifying laboratory aquifer columns. Appl Environ Microbiol. 1988 Feb;54(2):490–496. doi: 10.1128/aem.54.2.490-496.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovley D. R., Lonergan D. J. Anaerobic Oxidation of Toluene, Phenol, and p-Cresol by the Dissimilatory Iron-Reducing Organism, GS-15. Appl Environ Microbiol. 1990 Jun;56(6):1858–1864. doi: 10.1128/aem.56.6.1858-1864.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogel T. M., Grbìc-Galìc D. Incorporation of Oxygen from Water into Toluene and Benzene during Anaerobic Fermentative Transformation. Appl Environ Microbiol. 1986 Jul;52(1):200–202. doi: 10.1128/aem.52.1.200-202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeyer J., Kuhn E. P., Schwarzenbach R. P. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. doi: 10.1128/aem.52.4.944-947.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]