Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 Mar;58(3):877–883. doi: 10.1128/aem.58.3.877-883.1992

Isolation of New Aureobasidium Strains That Produce High-Molecular-Weight Pullulan with Reduced Pigmentation

Thomas J Pollock 1,*, Linda Thorne 1, Richard W Armentrout 1
PMCID: PMC195348  PMID: 16348676

Abstract

New isolates of Aureobasidium pullulans were obtained from plant leaf surfaces gathered in San Diego County. The new fungal isolates were identified as A. pullulans on the basis of the appearance of polymorphic colonies formed on agar plates, the electrophoretic profiles of repeated genomic DNA sequences, and the production of pullulan in shake flask cultures. The isolates showed different degrees of pigmentation. One of the natural isolates was nonpigmented under mock production conditions in liquid culture, but was still able to synthesize a reduced amount of pigment on agar plates at late times. A mutagenic treatment with ethidium bromide produced derivatives of normally pigmented natural isolates that exhibited an increased tendency toward yeastlike growth and reduced pigmentation. Additionally, some of the new isolates and mutant derivatives accumulated pullulan of relatively high molecular weight in the culture broths.

Full text

PDF
877

Images in this article

880Image
on p.880
881Image
on p.881
881Image
on p.881

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COOKE W. B. An ecological life history of Aureobasidium pullulans (De Bary) Arnaud. Mycopathol Mycol Appl. 1959 Dec 30;12:1–45. doi: 10.1007/BF02118435. [DOI] [PubMed] [Google Scholar]
  2. Catley B. J. Pullulan, a relationship between molecular weight and fine structure. FEBS Lett. 1970 Oct 5;10(3):190–193. doi: 10.1016/0014-5793(70)80450-1. [DOI] [PubMed] [Google Scholar]
  3. Imshenetskii A. A., Kondrat'eva T. F. Eksperimental'noe poluchenie poliploidnykh form u Pullularia pullulans. Dokl Akad Nauk SSSR. 1978;239(5):1235–1237. [PubMed] [Google Scholar]
  4. Kocková-Kratochvílová A., Cernáková M., Sláviková E. Morphological changes during the life cycle of Aureobasidium pullulans (de Bary) Arnaud. Folia Microbiol (Praha) 1980;25(1):56–67. doi: 10.1007/BF02876398. [DOI] [PubMed] [Google Scholar]
  5. Leathers T. D. Color Variants of Aureobasidium pullulans Overproduce Xylanase with Extremely High Specific Activity. Appl Environ Microbiol. 1986 Nov;52(5):1026–1030. doi: 10.1128/aem.52.5.1026-1030.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Magee B. B., D'Souza T. M., Magee P. T. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bacteriol. 1987 Apr;169(4):1639–1643. doi: 10.1128/jb.169.4.1639-1643.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Philippsen P., Stotz A., Scherf C. DNA of Saccharomyces cerevisiae. Methods Enzymol. 1991;194:169–182. doi: 10.1016/0076-6879(91)94014-4. [DOI] [PubMed] [Google Scholar]
  8. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]
  10. UEDA S., FUJITA K., KOMATSU K., NAKASHIMA Z. I. Polysaccharide produced by the genus Pullularia. I. Production of polysaccharide by growing cells. Appl Microbiol. 1963 May;11:211–215. doi: 10.1128/am.11.3.211-215.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Whelan W. L., Partridge R. M., Magee P. T. Heterozygosity and segregation in Candida albicans. Mol Gen Genet. 1980;180(1):107–113. doi: 10.1007/BF00267358. [DOI] [PubMed] [Google Scholar]
  12. Wickerham L. J., Kurtzman C. P. Synergistic color variants of Aureobasidium pullulans. Mycologia. 1975 Mar-Apr;67(2):342–361. [PubMed] [Google Scholar]
  13. Zajic J. E., LeDuy A. Flocculant and chemical properties of a polysaccharide from Pullularia pullulans. Appl Microbiol. 1973 Apr;25(4):628–635. doi: 10.1128/am.25.4.628-635.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES