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. 1992 Mar;58(3):1031–1034. doi: 10.1128/aem.58.3.1031-1034.1992

Molecular Cloning of the Tryptophan Operon from an Aeromonas hydrophila Freshwater Isolate

Juan Anguita 1, Octavio Rivero 1, Germán Naharro 1,*
PMCID: PMC195374  PMID: 16348662

Abstract

A genomic library of Aeromonas hydrophila F9 was constructed by using pBR322 as a vector. From that, two DNA fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpA and trpB defects (5.8-kb fragment) and to complement trpA, trpB, trpC, trpD, and trpE defects (11.6-kb fragment) in Escherichia coli mutants. Evidence of the existence of a secondary promoter is given.

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Selected References

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