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. 1992 Apr;58(4):1201–1206. doi: 10.1128/aem.58.4.1201-1206.1992

Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores

Mark T Hood 1,*, Chester Stachow 1
PMCID: PMC195575  PMID: 16348690

Abstract

The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustained until removal of PEG. In addition, the permeability of electroporated S. pombe envelopes is further increased with longer incubation times in PEG. The increased permeability is apparently a result of enlarged pores (electropores) due to the presence of PEG. Comparison of a heat pulse transformation protocol with electroporation suggests a second role for PEG in the uptake of macromolecules. Since pores are not thought to be created during a heat pulse, the PEG may be facilitating the uptake of plasmid DNA. This facilitation of uptake would also be expected to affect DNA uptake by electroporated cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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