Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 Apr;58(4):1276–1283. doi: 10.1128/aem.58.4.1276-1283.1992

Isolation of Pseudomonas pickettii strains that degrade 2,4,6-trichlorophenol and their dechlorination of chlorophenols.

H Kiyohara 1, T Hatta 1, Y Ogawa 1, T Kakuda 1, H Yokoyama 1, N Takizawa 1
PMCID: PMC195587  PMID: 1599247

Abstract

Three strains of Pseudomonas pickettii that can grow with 2,4,6-trichlorophenol (2,4,6-TCP) as the sole source of carbon and energy were isolated from different mixed cultures of soil bacterial populations that had been acclimatized to 2,4,6-TCP. These strains released 3 mol of chloride ion from 1 mol of 2,4,6-TCP during the complete degradation of the TCP. Of these strains, P. pickettii DTP0602 in high-cell-density suspension cultures dechlorinated various chlorophenols (CPs). Cells that were preincubated with 2,4,6-TCP converted isomers of 4-CP to the corresponding chloro-p-hydroquinones, but those preincubated with 4-CP converted CPs lacking a chlorine atom(s) at the o position to isomers of chlorocatechol. The ability of DTP0602 to dechlorinate 2,4,6-TCP was induced by 2,6-dichlorophenol, 2,3,6- and 2,4,6-TCP, and 2,3,4,6-tetrachlorophenol and was repressed in the presence of succinate or glucose.

Full text

PDF
1276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander M. Biodegradation: problems of molecular recalcitrance and microbial fallibility. Adv Appl Microbiol. 1965;7:35–80. doi: 10.1016/s0065-2164(08)70383-6. [DOI] [PubMed] [Google Scholar]
  2. Apajalahti J. H., Salkinoja-Salonen M. S. Complete dechlorination of tetrachlorohydroquinone by cell extracts of pentachlorophenol-induced Rhodococcus chlorophenolicus. J Bacteriol. 1987 Nov;169(11):5125–5130. doi: 10.1128/jb.169.11.5125-5130.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Apajalahti J. H., Salkinoja-Salonen M. S. Dechlorination and para-hydroxylation of polychlorinated phenols by Rhodococcus chlorophenolicus. J Bacteriol. 1987 Feb;169(2):675–681. doi: 10.1128/jb.169.2.675-681.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garcia J. L., Pichinoty F., Mandel M., Greenway B. A new denitrifying saprophyte related to Pseudomonas pickettii. Ann Microbiol (Paris) 1977 Feb-Mar;128(2):229–237. [PubMed] [Google Scholar]
  5. Karns J. S., Kilbane J. J., Duttagupta S., Chakrabarty A. M. Metabolism of Halophenols by 2,4,5-trichlorophenoxyacetic acid-degrading Pseudomonas cepacia. Appl Environ Microbiol. 1983 Nov;46(5):1176–1181. doi: 10.1128/aem.46.5.1176-1181.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kukor J. J., Olsen R. H. Molecular cloning, characterization, and regulation of a Pseudomonas pickettii PKO1 gene encoding phenol hydroxylase and expression of the gene in Pseudomonas aeruginosa PAO1c. J Bacteriol. 1990 Aug;172(8):4624–4630. doi: 10.1128/jb.172.8.4624-4630.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Li D. Y., Eberspächer J., Wagner B., Kuntzer J., Lingens F. Degradation of 2,4,6-trichlorophenol by Azotobacter sp. strain GP1. Appl Environ Microbiol. 1991 Jul;57(7):1920–1928. doi: 10.1128/aem.57.7.1920-1928.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. O'Reilly K. T., Crawford R. L. Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells. Appl Environ Microbiol. 1989 Sep;55(9):2113–2118. doi: 10.1128/aem.55.9.2113-2118.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ruckdeschel G., Renner G., Schwarz K. Effects of pentachlorophenol and some of its known and possible metabolites on different species of bacteria. Appl Environ Microbiol. 1987 Nov;53(11):2689–2692. doi: 10.1128/aem.53.11.2689-2692.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saber D. L., Crawford R. L. Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol. Appl Environ Microbiol. 1985 Dec;50(6):1512–1518. doi: 10.1128/aem.50.6.1512-1518.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sangodkar U. M., Chapman P. J., Chakrabarty A. M. Cloning, physical mapping and expression of chromosomal genes specifying degradation of the herbicide 2,4,5-T by Pseudomonas cepacia AC1100. Gene. 1988 Nov 30;71(2):267–277. doi: 10.1016/0378-1119(88)90043-1. [DOI] [PubMed] [Google Scholar]
  12. Schenk T., Müller R., Mörsberger F., Otto M. K., Lingens F. Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790. J Bacteriol. 1989 Oct;171(10):5487–5491. doi: 10.1128/jb.171.10.5487-5491.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spain J. C., Zylstra G. J., Blake C. K., Gibson D. T. Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl Environ Microbiol. 1989 Oct;55(10):2648–2652. doi: 10.1128/aem.55.10.2648-2652.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanlake G. J., Finn R. K. Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1982 Dec;44(6):1421–1427. doi: 10.1128/aem.44.6.1421-1427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steiert J. G., Crawford R. L. Catabolism of pentachlorophenol by a Flavobacterium sp. Biochem Biophys Res Commun. 1986 Dec 15;141(2):825–830. doi: 10.1016/s0006-291x(86)80247-9. [DOI] [PubMed] [Google Scholar]
  16. Steiert J. G., Pignatello J. J., Crawford R. L. Degradation of chlorinated phenols by a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1987 May;53(5):907–910. doi: 10.1128/aem.53.5.907-910.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Topp E., Crawford R. L., Hanson R. S. Influence of readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp. Appl Environ Microbiol. 1988 Oct;54(10):2452–2459. doi: 10.1128/aem.54.10.2452-2459.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Topp E., Hanson R. S. Degradation of pentachlorophenol by a Flavobacterium species grown in continuous culture under various nutrient limitations. Appl Environ Microbiol. 1990 Feb;56(2):541–544. doi: 10.1128/aem.56.2.541-544.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Xun L., Orser C. S. Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723. J Bacteriol. 1991 Jul;173(14):4447–4453. doi: 10.1128/jb.173.14.4447-4453.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES