Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 Apr;58(4):1379–1381. doi: 10.1128/aem.58.4.1379-1381.1992

Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase.

J Gaskell 1, A Vanden Wymelenberg 1, P Stewart 1, D Cullen 1
PMCID: PMC195604  PMID: 1599257

Abstract

A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.

Full text

PDF
1379

Images in this article

1380Image
on p.1380
1380Image
on p.1380

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alic M., Letzring C., Gold M. H. Mating System and Basidiospore Formation in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Appl Environ Microbiol. 1987 Jul;53(7):1464–1469. doi: 10.1128/aem.53.7.1464-1469.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrawis A., Pease E. A., Kuan I. C., Holzbaur E., Tien M. Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1989 Jul 31;162(2):673–680. doi: 10.1016/0006-291x(89)92363-2. [DOI] [PubMed] [Google Scholar]
  3. Brown A., Sims P. F., Raeder U., Broda P. Multiple ligninase-related genes from Phanerochaete chrysosporium. Gene. 1988 Dec 15;73(1):77–85. doi: 10.1016/0378-1119(88)90314-9. [DOI] [PubMed] [Google Scholar]
  4. Gaskell J., Dieperink E., Cullen D. Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium. Nucleic Acids Res. 1991 Feb 11;19(3):599–603. doi: 10.1093/nar/19.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huoponen K., Ollikka P., Kälin M., Walther I., Mäntsälä P., Reiser J. Characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes. Gene. 1990 Apr 30;89(1):145–150. doi: 10.1016/0378-1119(90)90218-g. [DOI] [PubMed] [Google Scholar]
  6. Kirk T. K., Farrell R. L. Enzymatic "combustion": the microbial degradation of lignin. Annu Rev Microbiol. 1987;41:465–505. doi: 10.1146/annurev.mi.41.100187.002341. [DOI] [PubMed] [Google Scholar]
  7. Mead D. A., Pey N. K., Herrnstadt C., Marcil R. A., Smith L. M. A universal method for the direct cloning of PCR amplified nucleic acid. Biotechnology (N Y) 1991 Jul;9(7):657–663. doi: 10.1038/nbt0791-657. [DOI] [PubMed] [Google Scholar]
  8. Naidu P. S., Reddy C. A. Nucleotide sequence of a new lignin peroxidase gene GLG3 from the white-rot fungus, Phanerochaete chrysosporium. Nucleic Acids Res. 1990 Dec 11;18(23):7173–7173. doi: 10.1093/nar/18.23.7173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schalch H., Gaskell J., Smith T. L., Cullen D. Molecular cloning and sequences of lignin peroxidase genes of Phanerochaete chrysosporium. Mol Cell Biol. 1989 Jun;9(6):2743–2747. doi: 10.1128/mcb.9.6.2743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith T. L., Schalch H., Gaskell J., Covert S., Cullen D. Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium. Nucleic Acids Res. 1988 Feb 11;16(3):1219–1219. doi: 10.1093/nar/16.3.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tien M., Tu C. P. Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium. Nature. 1987 Apr 2;326(6112):520–523. doi: 10.1038/326520a0. [DOI] [PubMed] [Google Scholar]
  13. Wallace R. B., Miyada C. G. Oligonucleotide probes for the screening of recombinant DNA libraries. Methods Enzymol. 1987;152:432–442. doi: 10.1016/0076-6879(87)52050-x. [DOI] [PubMed] [Google Scholar]
  14. Walther I., Kälin M., Reiser J., Suter F., Fritsche B., Saloheimo M., Leisola M., Teeri T., Knowles J. K., Fiechter A. Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene. Gene. 1988 Oct 15;70(1):127–137. doi: 10.1016/0378-1119(88)90111-4. [DOI] [PubMed] [Google Scholar]
  15. de Boer H. A., Zhang Y. Z., Collins C., Reddy C. A. Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus, Phanerochaete chrysosporium. Gene. 1987;60(1):93–102. doi: 10.1016/0378-1119(87)90217-4. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES