Abstract
Enterotoxin-positive strains of Clostridium perfringens were grown in Duncan-Strong sporulation medium in the presence of 0.4% (7.9 mM) raffinose at 37 and 43 degrees C. Enterotoxin- and heat-resistant spores were produced at similar concentrations but sooner at 43 degrees C than at 37 degrees C. There was a direct relationship between spore heat resistance and sporulation temperature (32, 37, and 43 degrees C).
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Duncan C. L., King G. J., Frieben W. R. A paracrystalline inclusion formed during sporulation of enterotoxin-producing strains of Clostridium perfringens type A. J Bacteriol. 1973 May;114(2):845–859. doi: 10.1128/jb.114.2.845-859.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- García-Alvarado J. S., Rodriguez M. A., Labbé R. G. Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A. Appl Environ Microbiol. 1992 Jan;58(1):326–330. doi: 10.1128/aem.58.1.326-330.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Granum P. E., Whitaker J. R. Improved method for purification of enterotoxin from Clostridium perfringens type A. Appl Environ Microbiol. 1980 Jun;39(6):1120–1122. doi: 10.1128/aem.39.6.1120-1122.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heredia N. L., Labbe R. G., Rodriguez M. A., Garcia-Alvarado J. S. Growth, sporulation and enterotoxin production by Clostridium perfringens type A in the presence of human bile salts. FEMS Microbiol Lett. 1991 Nov 1;68(1):15–21. doi: 10.1016/0378-1097(91)90387-p. [DOI] [PubMed] [Google Scholar]
- Kim C. H., Cheney R., Woodburn M. Sporulation of Clostridium perfringens in a modified medium and selected foods. Appl Microbiol. 1967 Jul;15(4):871–876. doi: 10.1128/am.15.4.871-876.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LECHOWICH R. V., ORDAL Z. J. The influence of the sporulation temperature on the heat resistance and chemical composition of bacterial spores. Can J Microbiol. 1962 Jun;8:287–295. doi: 10.1139/m62-040. [DOI] [PubMed] [Google Scholar]
- Labbe R. G., Duncan C. L. Sporulation and enterotoxin production by Clostridium perfringens type A under conditions of controlled pH and temperature. Can J Microbiol. 1974 Nov;20(11):1493–1501. doi: 10.1139/m74-233. [DOI] [PubMed] [Google Scholar]
- Labbe R. G., Nolan L. L. Stimulation of Clostridium perfringens enterotoxin formation by caffeine and theobromine. Infect Immun. 1981 Oct;34(1):50–54. doi: 10.1128/iai.34.1.50-54.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labbe R. G., Rey D. K. Raffinose increases sporulation and enterotoxin production by Clostridium perfringens type A. Appl Environ Microbiol. 1979 Jun;37(6):1196–1200. doi: 10.1128/aem.37.6.1196-1200.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Löffler A., Labbé R. Characterization of a parasporal inclusion body from sporulating, enterotoxin-positive Clostridium perfringens type A. J Bacteriol. 1986 Feb;165(2):542–548. doi: 10.1128/jb.165.2.542-548.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naik H. S., Duncan C. L. Rapid detection and quantitation of Clostridium perfringens enterostoxin by counterimmunoelectrophoresis. Appl Environ Microbiol. 1977 Aug;34(2):125–128. doi: 10.1128/aem.34.2.125-128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryu S., Labbe R. G. Coat and enterotoxin-related proteins in Clostridium perfringens spores. J Gen Microbiol. 1989 Nov;135(11):3109–3118. doi: 10.1099/00221287-135-11-3109. [DOI] [PubMed] [Google Scholar]
- Sacks L. E. Influence of carbohydrates on growth and sporulation of Clostridium perfringens in a defined medium with or without guanosine. Appl Environ Microbiol. 1983 Nov;46(5):1169–1175. doi: 10.1128/aem.46.5.1169-1175.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stark R. L., Duncan C. L. Biological characteristics of Clostridium perfringens type A enterotoxin. Infect Immun. 1971 Aug;4(2):89–96. doi: 10.1128/iai.4.2.89-96.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS O. B., ROBERTSON W. J. Studies on heat resistance. VI. Effect of temperature of incubation at which formed on heat resistance of aerobic thermophilic spores. J Bacteriol. 1954 Mar;67(3):377–378. doi: 10.1128/jb.67.3.377-378.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]

