Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 May;58(5):1490–1495. doi: 10.1128/aem.58.5.1490-1495.1992

Identification and cloning of Bradyrhizobium japonicum genes expressed strain selectively in soil and rhizosphere.

A A Bhagwat 1, D L Keister 1
PMCID: PMC195630  PMID: 1377899

Abstract

The growth of Bradyrhizobium japonicum USDA 110 and USDA 438 in soil extract-supplemented medium led to transcription of a large amount of DNA not expressed in basal medium. Strain USDA 438 was more competitive for the nodulation of soybean than strain USDA 110. To identify and isolate DNA regions which were expressed specifically in strain USDA 438 but not in strain USDA 110 in response to soil extract or soybean root exudate, we developed a subtractive RNA hybridization procedure. Several cosmid clones which showed strain-specific gene expression were isolated from a USDA 438 gene library. Two clones enhanced competitive nodulation when mobilized to USDA 110. The method described may be useful for identifying genes expressed in response to environmental stimuli or genes expressed differently in related microbial strains.

Full text

PDF
1490

Images in this article

1492Image
on p.1492
1492Image
on p.1492
1493Image
on p.1493
1493Image
on p.1493

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apte S. K., Haselkorn R. Cloning of salinity stress-induced genes from the salt-tolerant nitrogen-fixing cyanobacterium Anabaena torulosa. Plant Mol Biol. 1990 Nov;15(5):723–733. doi: 10.1007/BF00016122. [DOI] [PubMed] [Google Scholar]
  2. Bhagwat A. A., Thomas J. Legume-Rhizobium interactions: cowpea root exudate elicits faster nodulation response by Rhizobium species. Appl Environ Microbiol. 1982 Apr;43(4):800–805. doi: 10.1128/aem.43.4.800-805.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhagwat A. A., Tully R. E., Keister D. L. Isolation and Characterization of a Competition-Defective Bradyrhizobium japonicum Mutant. Appl Environ Microbiol. 1991 Dec;57(12):3496–3501. doi: 10.1128/aem.57.12.3496-3501.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caetano-Anollés G., Wall L. G., De Micheli A. T., Macchi E. M., Bauer W. D., Favelukes G. Role of Motility and Chemotaxis in Efficiency of Nodulation by Rhizobium meliloti. Plant Physiol. 1988 Apr;86(4):1228–1235. doi: 10.1104/pp.86.4.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cole M. A., Elkan G. H. Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum. Antimicrob Agents Chemother. 1973 Sep;4(3):248–253. doi: 10.1128/aac.4.3.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis E. O., Evans I. J., Johnston A. W. Identification of nodX, a gene that allows Rhizobium leguminosarum biovar viciae strain TOM to nodulate Afghanistan peas. Mol Gen Genet. 1988 Jun;212(3):531–535. doi: 10.1007/BF00330860. [DOI] [PubMed] [Google Scholar]
  7. Dowling D. N., Broughton W. J. Competition for nodulation of legumes. Annu Rev Microbiol. 1986;40:131–157. doi: 10.1146/annurev.mi.40.100186.001023. [DOI] [PubMed] [Google Scholar]
  8. Fuhrmann J. Symbiotic effectiveness of indigenous soybean bradyrhizobia as related to serological, morphological, rhizobitoxine, and hydrogenase phenotypes. Appl Environ Microbiol. 1990 Jan;56(1):224–229. doi: 10.1128/aem.56.1.224-229.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gubler M., Hennecke H. Regulation of the fixA gene and fixBC operon in Bradyrhizobium japonicum. J Bacteriol. 1988 Mar;170(3):1205–1214. doi: 10.1128/jb.170.3.1205-1214.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Göttfert M., Lamb J. W., Gasser R., Semenza J., Hennecke H. Mutational analysis of the Bradyrhizobium japonicum common nod genes and further nod box-linked genomic DNA regions. Mol Gen Genet. 1989 Feb;215(3):407–415. doi: 10.1007/BF00427037. [DOI] [PubMed] [Google Scholar]
  11. Hahn Matthias, Hennecke Hauke. Cloning and Mapping of a Novel Nodulation Region from Bradyrhizobium japonicum by Genetic Complementation of a Deletion Mutant. Appl Environ Microbiol. 1988 Jan;54(1):55–61. doi: 10.1128/aem.54.1.55-61.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Handelsman J., Ugalde R. A., Brill W. J. Rhizobium meliloti competitiveness and the alfalfa agglutinin. J Bacteriol. 1984 Mar;157(3):703–707. doi: 10.1128/jb.157.3.703-707.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hennecke H. Nitrogen fixation genes involved in the Bradyrhizobium japonicum-soybean symbiosis. FEBS Lett. 1990 Aug 1;268(2):422–426. doi: 10.1016/0014-5793(90)81297-2. [DOI] [PubMed] [Google Scholar]
  14. Keyser H. H., Cregan P. B. Nodulation and Competition for Nodulation of Selected Soybean Genotypes among Bradyrhizobium japonicum Serogroup 123 Isolates. Appl Environ Microbiol. 1987 Nov;53(11):2631–2635. doi: 10.1128/aem.53.11.2631-2635.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Keyser H. H., Weber D. F., Uratsu S. L. Rhizobium japonicum Serogroup and Hydrogenase Phenotype Distribution in 12 States. Appl Environ Microbiol. 1984 Apr;47(4):613–615. doi: 10.1128/aem.47.4.613-615.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Liu Ruilong, Tran Van Mai, Schmidt E. L. Nodulating Competitiveness of a Nonmotile Tn7 Mutant of Bradyrhizobium japonicum in Nonsterile Soil. Appl Environ Microbiol. 1989 Aug;55(8):1895–1900. doi: 10.1128/aem.55.8.1895-1900.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Long S. R. Rhizobium genetics. Annu Rev Genet. 1989;23:483–506. doi: 10.1146/annurev.ge.23.120189.002411. [DOI] [PubMed] [Google Scholar]
  18. McDermoti T. R., Graham P. H. Competitive Ability and Efficiency in Nodule Formation of Strains of Bradyrhizobium japonicum. Appl Environ Microbiol. 1990 Oct;56(10):3035–3039. doi: 10.1128/aem.56.10.3035-3039.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moawad H. A., Ellis W. R., Schmidt E. L. Rhizosphere Response as a Factor in Competition Among Three Serogroups of Indigenous Rhizobium japonicum for Nodulation of Field-Grown Soybeans. Appl Environ Microbiol. 1984 Apr;47(4):607–612. doi: 10.1128/aem.47.4.607-612.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sadowsky M. J., Cregan P. B., Gottfert M., Sharma A., Gerhold D., Rodriguez-Quinones F., Keyser H. H., Hennecke H., Stacey G. The Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeans. Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):637–641. doi: 10.1073/pnas.88.2.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sadowsky M. J., Cregan P. B., Keyser H. H. DNA Hybridization Probe for Use in Determining Restricted Nodulation among Bradyrhizobium japonicum Serocluster 123 Field Isolates. Appl Environ Microbiol. 1990 Jun;56(6):1768–1774. doi: 10.1128/aem.56.6.1768-1774.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sadowsky M. J., Tully R. E., Cregan P. B., Keyser H. H. Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean. Appl Environ Microbiol. 1987 Nov;53(11):2624–2630. doi: 10.1128/aem.53.11.2624-2630.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scott-Craig J. S., Guerinot M. L., Chelm B. K. Isolation of Bradyrhizobium japonicum DNA sequences that are transcribed at high levels in bacteroids. Mol Gen Genet. 1991 Sep;228(3):356–360. doi: 10.1007/BF00260627. [DOI] [PubMed] [Google Scholar]
  24. Triplett E. W. Construction of a Symbiotically Effective Strain of Rhizobium leguminosarum bv. trifolii with Increased Nodulation Competitiveness. Appl Environ Microbiol. 1990 Jan;56(1):98–103. doi: 10.1128/aem.56.1.98-103.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Viteri S. E., Schmidt E. L. Ecology of Indigenous Soil Rhizobia: Response of Bradyrhizobium japonicum to Readily Available Substrates. Appl Environ Microbiol. 1987 Aug;53(8):1872–1875. doi: 10.1128/aem.53.8.1872-1875.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wang S. P., Stacey G. Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box. J Bacteriol. 1991 Jun;173(11):3356–3365. doi: 10.1128/jb.173.11.3356-3365.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES