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. 1992 May;58(5):1624–1628. doi: 10.1128/aem.58.5.1624-1628.1992

Long-term starvation survival of Yersinia ruckeri at different salinities studied by microscopical and flow cytometric methods.

B K Thorsen 1, O Enger 1, S Norland 1, K A Hoff 1
PMCID: PMC195649  PMID: 1622232

Abstract

Cultures of three strains of the fish pathogenic bacterium Yersinia ruckeri survived starvation in unsupplemented water for at least 4 months. At salinities of 0 to 20/1000 there were no detectable changes in CFU during the first 3 days of starvation and only a small decrease during the following 4 months, whereas at 35/1000 salinity, the survival potential of the cultures was markedly reduced. These results suggest that Y. ruckeri may survive for long periods in freshwater and brackish environments after an outbreak of enteric redmouth disease. Survival was also examined by use of the direct viable count method, and we show that this method can be combined with flow cytometry for automatic counting of viable bacteria. By flow cytometry, it was shown that genome replication initiated before the onset of starvation was completed, during the initial phase of starvation, and that starved cells could contain up to six genomes per cell.

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Selected References

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  1. Amy P. S., Pauling C., Morita R. Y. Recovery from nutrient starvation by a marine Vibrio sp. Appl Environ Microbiol. 1983 May;45(5):1685–1690. doi: 10.1128/aem.45.5.1685-1690.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amy P. S., Pauling C., Morita R. Y. Starvation-survival processes of a marine Vibrio. Appl Environ Microbiol. 1983 Mar;45(3):1041–1048. doi: 10.1128/aem.45.3.1041-1048.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker R. M., Singleton F. L., Hood M. A. Effects of nutrient deprivation on Vibrio cholerae. Appl Environ Microbiol. 1983 Oct;46(4):930–940. doi: 10.1128/aem.46.4.930-940.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bjørnsen P. K. Automatic determination of bacterioplankton biomass by image analysis. Appl Environ Microbiol. 1986 Jun;51(6):1199–1204. doi: 10.1128/aem.51.6.1199-1204.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyaval P., Boyaval E., Desmazeaud M. J. Survival of Brevibacterium linens during nutrient starvation and intracellular changes. Arch Microbiol. 1985 Mar;141(2):128–132. doi: 10.1007/BF00423272. [DOI] [PubMed] [Google Scholar]
  6. Boye E., Steen H. B., Skarstad K. Flow cytometry of bacteria: a promising tool in experimental and clinical microbiology. J Gen Microbiol. 1983 Apr;129(4):973–980. doi: 10.1099/00221287-129-4-973. [DOI] [PubMed] [Google Scholar]
  7. Donnelly C. W., Baigent G. J. Method for flow cytometric detection of Listeria monocytogenes in milk. Appl Environ Microbiol. 1986 Oct;52(4):689–695. doi: 10.1128/aem.52.4.689-695.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grimes D. J., Atwell R. W., Brayton P. R., Palmer L. M., Rollins D. M., Roszak D. B., Singleton F. L., Tamplin M. L., Colwell R. R. The fate of enteric pathogenic bacteria in estuarine and marine environments. Microbiol Sci. 1986 Nov;3(11):324–329. [PubMed] [Google Scholar]
  9. Hoff K. A. Survival of Vibrio anguillarum and Vibrio salmonicida at different salinities. Appl Environ Microbiol. 1989 Jul;55(7):1775–1786. doi: 10.1128/aem.55.7.1775-1786.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kogure K., Koike I. Particle counter determination of bacterial biomass in seawater. Appl Environ Microbiol. 1987 Feb;53(2):274–277. doi: 10.1128/aem.53.2.274-277.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kogure K., Simidu U., Taga N. A tentative direct microscopic method for counting living marine bacteria. Can J Microbiol. 1979 Mar;25(3):415–420. doi: 10.1139/m79-063. [DOI] [PubMed] [Google Scholar]
  12. Munro P. M., Gauthier M. J., Laumond F. M. Changes in Escherichia coli cells starved in seawater or grown in seawater-wastewater mixtures. Appl Environ Microbiol. 1987 Jul;53(7):1476–1481. doi: 10.1128/aem.53.7.1476-1481.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novitsky J. A., Morita R. Y. Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine vibrio. Appl Environ Microbiol. 1976 Oct;32(4):617–622. doi: 10.1128/aem.32.4.617-622.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Novitsky J. A., Morita R. Y. Survival of a psychrophilic marine Vibrio under long-term nutrient starvation. Appl Environ Microbiol. 1977 Mar;33(3):635–641. doi: 10.1128/aem.33.3.635-641.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rollins D. M., Colwell R. R. Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl Environ Microbiol. 1986 Sep;52(3):531–538. doi: 10.1128/aem.52.3.531-538.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roszak D. B., Colwell R. R. Survival strategies of bacteria in the natural environment. Microbiol Rev. 1987 Sep;51(3):365–379. doi: 10.1128/mr.51.3.365-379.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Scherer C. G., Boylen C. W. Macromolecular synthesis and degradation in Arthrobacter during periods of nutrient deprivation. J Bacteriol. 1977 Nov;132(2):584–589. doi: 10.1128/jb.132.2.584-589.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Singh A., Pyle B. H., McFeters G. A. Rapid enumeration of viable bacteria by image analysis. J Microbiol Methods. 1989;10:91–101. doi: 10.1016/0167-7012(89)90005-5. [DOI] [PubMed] [Google Scholar]
  19. Steen H. B., Lindmo T. Flow cytometry: a high-resolution instrument for everyone. Science. 1979 Apr 27;204(4391):403–404. doi: 10.1126/science.441727. [DOI] [PubMed] [Google Scholar]
  20. Tabor P. S., Neihof R. A. Direct determination of activities for microorganisms of chesapeake bay populations. Appl Environ Microbiol. 1984 Nov;48(5):1012–1019. doi: 10.1128/aem.48.5.1012-1019.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Völsch A., Nader W. F., Geiss H. K., Nebe G., Birr C. Detection and analysis of two serotypes of ammonia-oxidizing bacteria in sewage plants by flow cytometry. Appl Environ Microbiol. 1990 Aug;56(8):2430–2435. doi: 10.1128/aem.56.8.2430-2435.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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