Abstract
Tannin-protein complex (T-PC)-degrading enterobacteria (T-PCDE) were isolated from the feces and from a layer of bacteria attached to the cecal wall of koalas. The T-PCDE were facultatively anaerobic, gram-negative, pleomorphic, nonmotile bacilli. The bacteria were also oxidase and catalase negative and resistant to vancomycin, reduced nitrates to nitrites, and grew on MacConkey agar. Growth on tannin-treated agar media showed a distinctive clear zone around the colony. From these observations, a selective agar plate medium (vancomycin- and tannin-treated Wilkins-Chalgren anaerobe agar) was developed to enumerate T-PCDE isolated from the feces of koalas. This medium was highly selective in the enumeration of the fecal T-PCDE and inhibited the growth of concomitant T-PC-degrading Streptococcus bovis. The T-PCDE were isolated from 10 of the 12 captive koalas studied; in 8 of these 10 koalas, the facultatively anaerobic bacterial flora was dominated (more than 60%) by T-PCDE. Viable numbers of T-PCDE were, in most of the animals, much larger (more than 100 times) than the numbers of T-PC-degrading S. bovis, suggesting that T-PCDE played a more active role in digesting T-PC in the alimentary tracts of koalas.
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- Graham D. C., Hodgkiss W. Identity of gram negative, yellow pigmented, fermentative bacteria isolated from plants and animals. J Appl Bacteriol. 1967 Apr;30(1):175–189. doi: 10.1111/j.1365-2672.1967.tb00287.x. [DOI] [PubMed] [Google Scholar]
- Osawa R. Formation of a clear zone on tannin-treated brain heart infusion agar by a Streptococcus sp. isolated from feces of koalas. Appl Environ Microbiol. 1990 Mar;56(3):829–831. doi: 10.1128/aem.56.3.829-831.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osawa R., Mitsuoka T. Selective Medium for Enumeration of Tannin-Protein Complex-Degrading Streptococcus spp. in Feces of Koalas. Appl Environ Microbiol. 1990 Nov;56(11):3609–3611. doi: 10.1128/aem.56.11.3609-3611.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pien F. D., Martin W. J., Hermans P. E., Washington J. A., 2nd Clinical and bacteriologic observations on the proposed species, Enterobacter agglomerans (the Herbicola-Lathyri bacteria). Mayo Clin Proc. 1972 Oct;47(10):739–745. [PubMed] [Google Scholar]
- Potrikus C. J., Breznak J. A. Nitrogen-fixing Enterobacter agglomerans isolated from guts of wood-eating termites. Appl Environ Microbiol. 1977 Feb;33(2):392–399. doi: 10.1128/aem.33.2.392-399.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]