Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1992 May;58(5):1772–1775. doi: 10.1128/aem.58.5.1772-1775.1992

Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labels.

M R Cancilla 1, I B Powell 1, A J Hillier 1, B E Davidson 1
PMCID: PMC195671  PMID: 1622250

Abstract

Arbitrarily primed polymerase chain reaction, with incorporation of either radioactive or fluorescent labels, was used as a rapid and sensitive method for obtaining genomic fingerprints of strains of Lactococcus lactis. Closely related strains produced almost identical fingerprints. Fingerprints of other strains showed only some similarities.

Full text

PDF

Images in this article

1773Image
on p.1773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Betzl D., Ludwig W., Schleifer K. H. Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol. 1990 Sep;56(9):2927–2929. doi: 10.1128/aem.56.9.2927-2929.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davey G. P., Richardson B. C. Purification and Some Properties of Diplococcin from Streptococcus cremoris 346. Appl Environ Microbiol. 1981 Jan;41(1):84–89. doi: 10.1128/aem.41.1.84-89.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Efstathiou J. D., McKay L. L. Inorganic salts resistance associated with a lactose-fermenting plasmid in Streptococcus lactis. J Bacteriol. 1977 Apr;130(1):257–265. doi: 10.1128/jb.130.1.257-265.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Le Bourgeois P., Mata M., Ritzenthaler P. Genome comparison of Lactococcus strains by pulsed-field gel electrophoresis. FEMS Microbiol Lett. 1989 May;50(1-2):65–69. doi: 10.1016/0378-1097(89)90460-6. [DOI] [PubMed] [Google Scholar]
  5. Salama M., Sandine W., Giovannoni S. Development and application of oligonucleotide probes for identification of Lactococcus lactis subsp. cremoris. Appl Environ Microbiol. 1991 May;57(5):1313–1318. doi: 10.1128/aem.57.5.1313-1318.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Tanskanen E. I., Tulloch D. L., Hillier A. J., Davidson B. E. Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification. Appl Environ Microbiol. 1990 Oct;56(10):3105–3111. doi: 10.1128/aem.56.10.3105-3111.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tulloch D. L., Finch L. R., Hillier A. J., Davidson B. E. Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons. J Bacteriol. 1991 May;173(9):2768–2775. doi: 10.1128/jb.173.9.2768-2775.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Welsh J., Petersen C., McClelland M. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Res. 1991 Jan 25;19(2):303–306. doi: 10.1093/nar/19.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Williams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. doi: 10.1093/nar/18.22.6531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES