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. 1992 Jun;58(6):1797–1800. doi: 10.1128/aem.58.6.1797-1800.1992

Preparation of Refractile Spores of Clostridium thermosaccharolyticum Involves a Solventogenic Phase

Sandra L Landuyt 1,†, Edward J Hsu 1,*
PMCID: PMC195686  PMID: 16348716

Abstract

Conversion of vegetative cells of Clostridium thermosaccharolyticum to refractile endospores was achieved by sequential transfer and dilution at each generation, with a final dilution into a sporulation medium that contained xylan supplemented with excess calcium. The subsequent growth was synchronous and resulted in elongated, solventogenic cells that were then shifted to 35°C to permit further differentiation without cell division. The synchronized cells grown in xylan medium supplemented with Ca gluconate produced total solvents that reached 9.63% (vol/vol). One hundred percent of these elongated solventogenic cells (4.84 × 109 cells per ml) entered the sporangial stage and continued to differentiate into refractile spores. Only cells sequentially transferred and diluted at a critical time of the growth cycle are synchronized, induced to elongate (≥fourfold), become highly solventogenic in the presence of excess calcium, and are converted to a homogeneous population of refractile spores.

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Selected References

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