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. 1992 Aug;58(8):2704–2707. doi: 10.1128/aem.58.8.2704-2707.1992

Temperature-dependent induction of an acid-inducible stimulon of Escherichia coli in broth.

M Hassani 1, D H Pincus 1, G N Bennett 1, I N Hirshfield 1
PMCID: PMC195847  PMID: 1514820

Abstract

The induction of the inducible lysyl-tRNA synthetase, LysU, and the inducible lysine and arginine decarboxylases of Escherichia coli K-12 grown in AC broth to a pH of 5.5 or less is temperature dependent, being distinctly lower at 24 than at 37 degrees C. This induction does not appear to be under HtpR control.

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Selected References

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  1. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Foster J. W., Hall H. K. Adaptive acidification tolerance response of Salmonella typhimurium. J Bacteriol. 1990 Feb;172(2):771–778. doi: 10.1128/jb.172.2.771-778.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Foster J. W., Hall H. K. Inducible pH homeostasis and the acid tolerance response of Salmonella typhimurium. J Bacteriol. 1991 Aug;173(16):5129–5135. doi: 10.1128/jb.173.16.5129-5135.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gale E. F., Epps H. M. The effect of the pH of the medium during growth on the enzymic activities of bacteria (Escherichia coli and Micrococcus lysodeikticus) and the biological significance of the changes produced. Biochem J. 1942 Sep;36(7-9):600–618. doi: 10.1042/bj0360600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grossman A. D., Erickson J. W., Gross C. A. The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984 Sep;38(2):383–390. doi: 10.1016/0092-8674(84)90493-8. [DOI] [PubMed] [Google Scholar]
  6. Hassani M., Saluta M. V., Bennett G. N., Hirshfield I. N. Partial characterization of a lysU mutant of Escherichia coli K-12. J Bacteriol. 1991 Mar;173(6):1965–1970. doi: 10.1128/jb.173.6.1965-1970.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heyde M., Portalier R. Acid shock proteins of Escherichia coli. FEMS Microbiol Lett. 1990 May;57(1-2):19–26. doi: 10.1016/0378-1097(90)90406-g. [DOI] [PubMed] [Google Scholar]
  8. Hickey E. W., Hirshfield I. N. Low-pH-induced effects on patterns of protein synthesis and on internal pH in Escherichia coli and Salmonella typhimurium. Appl Environ Microbiol. 1990 Apr;56(4):1038–1045. doi: 10.1128/aem.56.4.1038-1045.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirshfield I. N., Bloch P. L., Van Bogelen R. A., Neidhardt F. C. Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli. J Bacteriol. 1981 Apr;146(1):345–351. doi: 10.1128/jb.146.1.345-351.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirshfield I. N., Liu C., Yeh F. M. Two modes of metabolic regulation of lysyl-transfer ribonucleic acid synthetase in Escherichia coli K-12. J Bacteriol. 1977 Aug;131(2):589–597. doi: 10.1128/jb.131.2.589-597.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hirshfield I. N., Tenreiro R., Vanbogelen R. A., Neidhardt F. C. Escherichia coli K-12 lysyl-tRNA synthetase mutant with a novel reversion pattern. J Bacteriol. 1984 May;158(2):615–620. doi: 10.1128/jb.158.2.615-620.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lévêque F., Gazeau M., Fromant M., Blanquet S., Plateau P. Control of Escherichia coli lysyl-tRNA synthetase expression by anaerobiosis. J Bacteriol. 1991 Dec;173(24):7903–7910. doi: 10.1128/jb.173.24.7903-7910.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mallett A. K., Bearne C. A., Rowland I. R. The influence of incubation pH on the activity of rat and human gut flora enzymes. J Appl Bacteriol. 1989 May;66(5):433–437. doi: 10.1111/j.1365-2672.1989.tb05112.x. [DOI] [PubMed] [Google Scholar]
  15. NOVICK R. P., MAAS W. K. Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli. J Bacteriol. 1961 Feb;81:236–240. doi: 10.1128/jb.81.2.236-240.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neidhardt F. C., VanBogelen R. A. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem Biophys Res Commun. 1981 May 29;100(2):894–900. doi: 10.1016/s0006-291x(81)80257-4. [DOI] [PubMed] [Google Scholar]
  17. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  18. Raja N., Goodson M., Chui W. C., Smith D. G., Rowbury R. J. Habituation to acid in Escherichia coli: conditions for habituation and its effects on plasmid transfer. J Appl Bacteriol. 1991 Jan;70(1):59–65. doi: 10.1111/j.1365-2672.1991.tb03787.x. [DOI] [PubMed] [Google Scholar]
  19. Slonczewski J. L., Gonzalez T. N., Bartholomew F. M., Holt N. J. Mu d-directed lacZ fusions regulated by low pH in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3001–3006. doi: 10.1128/jb.169.7.3001-3006.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. White S., Tuttle F. E., Blankenhorn D., Dosch D. C., Slonczewski J. L. pH dependence and gene structure of inaA in Escherichia coli. J Bacteriol. 1992 Mar;174(5):1537–1543. doi: 10.1128/jb.174.5.1537-1543.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

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