Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Dec;59(12):4313–4316. doi: 10.1128/aem.59.12.4313-4316.1993

Antimicrobial activity of a newly identified bacteriocin of Bacillus cereus.

G Naclerio 1, E Ricca 1, M Sacco 1, M De Felice 1
PMCID: PMC195902  PMID: 8285719

Abstract

A bacteriocin-producing Bacillus cereus strain was isolated. The bacteriocin, here called cerein, was shown to be active specifically against other B. cereus strains and inactive against all other bacterial species tested. Cerein was detected in the culture supernatants of stationary-phase cells, and its appearance was inhibited by induction of sporulation. The bacterial activity of cerein was insensitive to organic solvents and nonproteolytic enzymes, partially stable to heat, and active over a wide range of pH values. Direct detection of antimicrobial activity on sodium dodecyl sulfate-polyacrylamide gel suggested an apparent molecular mass of about 9 kDa.

Full text

PDF
4313

Images in this article

4315Image
on p.4315

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee C., Bustamante C. I., Wharton R., Talley E., Wade J. C. Bacillus infections in patients with cancer. Arch Intern Med. 1988 Aug;148(8):1769–1774. [PubMed] [Google Scholar]
  2. Beecher D. J., MacMillan J. D. A novel bicomponent hemolysin from Bacillus cereus. Infect Immun. 1990 Jul;58(7):2220–2227. doi: 10.1128/iai.58.7.2220-2227.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beecher D. J., Macmillan J. D. Characterization of the components of hemolysin BL from Bacillus cereus. Infect Immun. 1991 May;59(5):1778–1784. doi: 10.1128/iai.59.5.1778-1784.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernheimer A. W., Grushoff P. Cereolysin: production, purification and partial characterization. J Gen Microbiol. 1967 Jan;46(1):143–150. doi: 10.1099/00221287-46-1-143. [DOI] [PubMed] [Google Scholar]
  5. Bhunia A. K., Johnson M. C., Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol. 1988 Oct;65(4):261–268. doi: 10.1111/j.1365-2672.1988.tb01893.x. [DOI] [PubMed] [Google Scholar]
  6. Christiansson A., Naidu A. S., Nilsson I., Wadström T., Pettersson H. E. Toxin production by Bacillus cereus dairy isolates in milk at low temperatures. Appl Environ Microbiol. 1989 Oct;55(10):2595–2600. doi: 10.1128/aem.55.10.2595-2600.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Funada H., Uotani C., Machi T., Matsuda T., Nonomura A. Bacillus cereus bacteremia in an adult with acute leukemia. Jpn J Clin Oncol. 1988 Mar;18(1):69–74. doi: 10.1093/jjco/18.1.69. [DOI] [PubMed] [Google Scholar]
  8. Gilmore M. S., Cruz-Rodz A. L., Leimeister-Wächter M., Kreft J., Goebel W. A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage. J Bacteriol. 1989 Feb;171(2):744–753. doi: 10.1128/jb.171.2.744-753.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson C. E., Bonventre P. F. Lethal toxin of Bacillus cereus. I. Relationships and nature of toxin, hemolysin, and phospholipase. J Bacteriol. 1967 Aug;94(2):306–316. doi: 10.1128/jb.94.2.306-316.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klaenhammer T. R. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. doi: 10.1016/0300-9084(88)90206-4. [DOI] [PubMed] [Google Scholar]
  11. Kreft J., Berger H., Härtlein M., Müller B., Weidinger G., Goebel W. Cloning and expression in Escherichia coli and Bacillus subtilis of the hemolysin (cereolysin) determinant from Bacillus cereus. J Bacteriol. 1983 Aug;155(2):681–689. doi: 10.1128/jb.155.2.681-689.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lindgren S. E., Dobrogosz W. J. Antagonistic activities of lactic acid bacteria in food and feed fermentations. FEMS Microbiol Rev. 1990 Sep;7(1-2):149–163. doi: 10.1111/j.1574-6968.1990.tb04885.x. [DOI] [PubMed] [Google Scholar]
  14. Mortimer P. R., McCann G. Food-poisoning episodes associated with Bacillus cereus in fried rice. Lancet. 1974 May 25;1(7865):1043–1045. doi: 10.1016/s0140-6736(74)90434-6. [DOI] [PubMed] [Google Scholar]
  15. Schofield G. M. Emerging food-borne pathogens and their significance in chilled foods. J Appl Bacteriol. 1992 Apr;72(4):267–273. doi: 10.1111/j.1365-2672.1992.tb01834.x. [DOI] [PubMed] [Google Scholar]
  16. Stoffels G., Nissen-Meyer J., Gudmundsdottir A., Sletten K., Holo H., Nes I. F. Purification and characterization of a new bacteriocin isolated from a Carnobacterium sp. Appl Environ Microbiol. 1992 May;58(5):1417–1422. doi: 10.1128/aem.58.5.1417-1422.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thompson N. E., Ketterhagen M. J., Bergdoll M. S., Schantz E. J. Isolation and some properties of an enterotoxin produced by Bacillus cereus. Infect Immun. 1984 Mar;43(3):887–894. doi: 10.1128/iai.43.3.887-894.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wong H. C., Chang M. H., Fan J. Y. Incidence and characterization of Bacillus cereus isolates contaminating dairy products. Appl Environ Microbiol. 1988 Mar;54(3):699–702. doi: 10.1128/aem.54.3.699-702.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Netten P., van De Moosdijk A., van Hoensel P., Mossel D. A., Perales I. Psychrotrophic strains of Bacillus cereus producing enterotoxin. J Appl Bacteriol. 1990 Jul;69(1):73–79. doi: 10.1111/j.1365-2672.1990.tb02913.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES