Abstract
The conditions for optimal steam decontamination of polypropylene bags half loaded with laboratory biomedical waste were studied (276 bags were processed). Controls were single-closed bags without water added or incisions made in the top, standing freely in an autoclave set at 121°C. The average time required to reach 121°C at the load center was 46 min for controls. A significant increase in this time occurred following addition of water to bags without incisions (60 min), with double bagging (60 min), or when using vertical containers (82 min). A significant decrease occurred when bags were slashed (37 min) or processed at 123°C (32 min) or 132°C (19 min). Horizontal containers or addition of water to slashed bags had no significant effect.
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Selected References
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- Gillespie E. H., Gibbons S. A. Autoclaves and their dangers and safety in laboratories. J Hyg (Lond) 1975 Dec;75(3):475–487. doi: 10.1017/s0022172400024517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lauer J. L., Battles D. R., Vesley D. Decontaminating infectious laboratory waste by autoclaving. Appl Environ Microbiol. 1982 Sep;44(3):690–694. doi: 10.1128/aem.44.3.690-694.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutala W. A., Stiegel M. M., Sarubbi F. A., Jr Decontamination of laboratory microbiological waste by steam sterilization. Appl Environ Microbiol. 1982 Jun;43(6):1311–1316. doi: 10.1128/aem.43.6.1311-1316.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]