Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Dec;59(12):4347–4349. doi: 10.1128/aem.59.12.4347-4349.1993

Rapid glutamate decarboxylase assay for detection of Escherichia coli.

E W Rice 1, C H Johnson 1, M E Dunnigan 1, D J Reasoner 1
PMCID: PMC195909  PMID: 8285721

Abstract

A rapid test procedure for the enzyme glutamate decarboxylase was developed for detection of Escherichia coli. The assay procedure was able to confirm the presence of E. coli in enteric broth cultures with 95% specificity for both pure cultures and environmental samples. The procedure was capable of detecting survivors among chlorine-exposed cells.

Full text

PDF
4347

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman D., Rice E. W., Hoff J. C. Inactivation of particle-associated coliforms by chlorine and monochloramine. Appl Environ Microbiol. 1988 Feb;54(2):507–512. doi: 10.1128/aem.54.2.507-512.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fiedler J., Eiske J. R. Glutaminsäuredecarboxylase-Schnelltest zur Identifikation von Escherichia coli. Z Gesamte Hyg. 1990 Nov;36(11):620–622. [PubMed] [Google Scholar]
  3. Freier P. A., Graves M. H., Kocka F. E. A rapid glutamic decarboxylase test for identification of bacteria. Ann Clin Lab Sci. 1976 Nov-Dec;6(6):537–539. [PubMed] [Google Scholar]
  4. Jilly B. J., Schreckenberger P. C., LeBeau L. J. Rapid glutamic acid decarboxylase test for identification of Bacteroides and Clostridium spp. J Clin Microbiol. 1984 May;19(5):592–593. doi: 10.1128/jcm.19.5.592-593.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schubert R., Esanu J. G., Schäfer V. Der Glutaminsäuredecarboxylase-Plättchen-Test. Ein Ansatz zur Vereinfachung und Beschleunigung des E. coli-Nachweises. Zentralbl Bakteriol Mikrobiol Hyg B. 1988 Dec;187(2):107–111. [PubMed] [Google Scholar]
  6. Shadix L. C., Rice E. W. Evaluation of beta-glucuronidase assay for the detection of Escherichia coli from environmental waters. Can J Microbiol. 1991 Dec;37(12):908–911. doi: 10.1139/m91-157. [DOI] [PubMed] [Google Scholar]
  7. Wauters G., Cornelis G. Méthode simple pour la recherche de la décarboxylation de l'acide glutamique chez les bactéries à gram négatif. Ann Microbiol (Paris) 1974 Feb-Mar;125A(2):183–192. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES