Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Dec;59(12):4361–4362. doi: 10.1128/aem.59.12.4361-4362.1993

Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts

A T Campbell 1, L J Robertson 1, H V Smith 1,*
PMCID: PMC195912  PMID: 16349135

Abstract

Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.

Full text

PDF
4361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campbell A. T., Robertson L. J., Smith H. V. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl Environ Microbiol. 1992 Nov;58(11):3488–3493. doi: 10.1128/aem.58.11.3488-3493.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Campbell I., Tzipori A. S., Hutchison G., Angus K. W. Effect of disinfectants on survival of cryptosporidium oocysts. Vet Rec. 1982 Oct 30;111(18):414–415. doi: 10.1136/vr.111.18.414. [DOI] [PubMed] [Google Scholar]
  3. Robertson L. J., Campbell A. T., Smith H. V. In vitro excystation of Cryptosporidium parvum. Parasitology. 1993 Jan;106(Pt 1):13–19. doi: 10.1017/s003118200007476x. [DOI] [PubMed] [Google Scholar]
  4. Robertson L. J., Campbell A. T., Smith H. V. Survival of Cryptosporidium parvum oocysts under various environmental pressures. Appl Environ Microbiol. 1992 Nov;58(11):3494–3500. doi: 10.1128/aem.58.11.3494-3500.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schmid I., Uittenbogaart C. H., Giorgi J. V. A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification. Cytometry. 1991;12(3):279–285. doi: 10.1002/cyto.990120312. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES