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. 1993 Dec;59(12):4363–4366. doi: 10.1128/aem.59.12.4363-4366.1993

Minimal Requirements for Exponential Growth of Lactococcus lactis

Peter Ruhdal Jensen 2, Karin Hammer 2,*
PMCID: PMC195913  PMID: 16349136

Abstract

A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of μ = 0.3 h-1. With 19 amino acids added, the growth rate increased to μ = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

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Selected References

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