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. 1993 Jan;175(2):503–509. doi: 10.1128/jb.175.2.503-509.1993

Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.

R Rudner 1, A Chevrestt 1, S R Buchholz 1, B Studamire 1, A M White 1, E D Jarvis 1
PMCID: PMC196165  PMID: 8419296

Abstract

Sequence analysis of cloned rescued DNA fragments from a Bacillus subtilis strain with an inserted recombinant plasmid in ribosomal operon rrnE revealed the presence of two tRNA genes for Met and Asp at the 3' end of the operon. Probing chromosomal DNA from a strain carrying a plasmid inserted in rrnD with a fragment containing the genetically unassigned cluster of 16 tRNA genes revealed that the cluster is located immediately following the rrnD operon. Our findings show that all 10 rrn operons in B. subtilis are associated with tRNA gene clusters.

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Selected References

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  1. Armstrong R. L., Sueoka N. Phase transitions in ribonucleic acid synthesis during germination of Bacillus subtilis spores. Proc Natl Acad Sci U S A. 1968 Jan;59(1):153–160. doi: 10.1073/pnas.59.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dedonder R. A., Lepesant J. A., Lepesant-Kejzlarová J., Billault A., Steinmetz M., Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. doi: 10.1128/aem.33.4.989-993.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferrari F. A., Nguyen A., Lang D., Hoch J. A. Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1513–1515. doi: 10.1128/jb.154.3.1513-1515.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fournier M. J., Ozeki H. Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12. Microbiol Rev. 1985 Dec;49(4):379–397. doi: 10.1128/mr.49.4.379-397.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green C. J., Stewart G. C., Hollis M. A., Vold B. S., Bott K. F. Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB. Gene. 1985;37(1-3):261–266. doi: 10.1016/0378-1119(85)90281-1. [DOI] [PubMed] [Google Scholar]
  6. Green C. J., Vold B. S. A cluster of nine tRNA genes between ribosomal gene operons in Bacillus subtilis. J Bacteriol. 1992 May;174(10):3147–3151. doi: 10.1128/jb.174.10.3147-3151.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henner D. J., Steinberg W. Genetic location of the Bacillus subtilis sup-3 suppressor mutation. J Bacteriol. 1979 Aug;139(2):668–670. doi: 10.1128/jb.139.2.668-670.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jarvis E. D., Cheng S., Rudner R. Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation. Genetics. 1990 Dec;126(4):785–797. doi: 10.1093/genetics/126.4.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jarvis E. D., Widom R. L., LaFauci G., Setoguchi Y., Richter I. R., Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics. 1988 Nov;120(3):625–635. doi: 10.1093/genetics/120.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Komine Y., Adachi T., Inokuchi H., Ozeki H. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J Mol Biol. 1990 Apr 20;212(4):579–598. doi: 10.1016/0022-2836(90)90224-A. [DOI] [PubMed] [Google Scholar]
  11. LaFauci G., Widom R. L., Eisner R. L., Jarvis E. D., Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol. 1986 Jan;165(1):204–214. doi: 10.1128/jb.165.1.204-214.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lipsky R. H., Rosenthal R., Zahler S. A. Defective specialized SP beta transducing bacteriophages of Bacillus subtilis that carry the sup-3 or sup-44 gene. J Bacteriol. 1981 Dec;148(3):1012–1015. doi: 10.1128/jb.148.3.1012-1015.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Loughney K., Lund E., Dahlberg J. E. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis. Nucleic Acids Res. 1982 Mar 11;10(5):1607–1624. doi: 10.1093/nar/10.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Margulies L., Setoguchi Y., Rudner R. Asymmetric transcription during post-germinative development of Bacillus subtilis spores. Biochim Biophys Acta. 1978 Dec 21;521(2):708–718. doi: 10.1016/0005-2787(78)90310-6. [DOI] [PubMed] [Google Scholar]
  15. Ogasawara N. Markedly unbiased codon usage in Bacillus subtilis. Gene. 1985;40(1):145–150. doi: 10.1016/0378-1119(85)90035-6. [DOI] [PubMed] [Google Scholar]
  16. Ogasawara N., Moriya S., Yoshikawa H. Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome. Nucleic Acids Res. 1983 Sep 24;11(18):6301–6318. doi: 10.1093/nar/11.18.6301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ogasawara N., Seiki M., Yoshikawa H. Replication origin region of Bacillus subtilis chromosome contains two rRNA operons. J Bacteriol. 1983 Apr;154(1):50–57. doi: 10.1128/jb.154.1.50-57.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Setoguchi Y., Margulies L., Rudner R. Asymmetric transcription during post-germinative development of Bacillus subtilis spores. II. Hybrid competition analyses. Biochim Biophys Acta. 1978 Dec 21;521(2):719–725. doi: 10.1016/0005-2787(78)90311-8. [DOI] [PubMed] [Google Scholar]
  19. Stewart G. C., Wilson F. E., Bott K. F. Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis. Gene. 1982 Sep;19(2):153–162. doi: 10.1016/0378-1119(82)90001-4. [DOI] [PubMed] [Google Scholar]
  20. Vold B. S., Green C. J., Narasimhan N., Strem M., Hansen J. N. Transcriptional analysis of Bacillus subtilis rRNA-tRNA operons. II. Unique properties of an operon containing a minor 5 S rRNA gene. J Biol Chem. 1988 Oct 5;263(28):14485–14490. [PubMed] [Google Scholar]
  21. Vold B. S., Okamoto K., Murphy B. J., Green C. J. Transcriptional analysis of Bacillus subtilis rRNA-tRNA operons. I. The tRNA gene cluster of rrnB has an internal promoter. J Biol Chem. 1988 Oct 5;263(28):14480–14484. [PubMed] [Google Scholar]
  22. Vold B. S. Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis. Microbiol Rev. 1985 Mar;49(1):71–80. doi: 10.1128/mr.49.1.71-80.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wawrousek E. F., Hansen J. N. Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis. J Biol Chem. 1983 Jan 10;258(1):291–298. [PubMed] [Google Scholar]
  24. Wawrousek E. F., Narasimhan N., Hansen J. N. Two large clusters with thirty-seven transfer RNA genes adjacent to ribosomal RNA gene sets in Bacillus subtilis. Sequence and organization of trrnD and trrnE gene clusters. J Biol Chem. 1984 Mar 25;259(6):3694–3702. [PubMed] [Google Scholar]
  25. Widom R. L., Jarvis E. D., LaFauci G., Rudner R. Instability of rRNA operons in Bacillus subtilis. J Bacteriol. 1988 Feb;170(2):605–610. doi: 10.1128/jb.170.2.605-610.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yamada Y., Ohki M., Ishikura H. The nucleotide sequence of Bacillus subtilis tRNA genes. Nucleic Acids Res. 1983 May 25;11(10):3037–3045. doi: 10.1093/nar/11.10.3037. [DOI] [PMC free article] [PubMed] [Google Scholar]

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