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. 1993 Jan;175(2):565–567. doi: 10.1128/jb.175.2.565-567.1993

Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis.

W Seol 1, A J Shatkin 1
PMCID: PMC196175  PMID: 8419306

Abstract

Escherichia coli alpha-ketoglutarate permease (KgtP) is a 432-amino-acid protein that symports alpha-ketoglutarate and protons. KgtP was predicted to contain 12 membrane-spanning domains on the basis of a calculated hydropathy profile. The membrane topology model of KgtP was analyzed by using kgtP-phoA gene fusions and measuring alkaline phosphatase activities in cells expressing the chimeric proteins. Comparisons of the phosphatase activity levels and the locations of the KgtP-PhoA junctions are consistent with the predicted membrane topology model of KgtP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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